Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22442 | 3' | -56.2 | NC_005056.1 | + | 3965 | 0.76 | 0.121031 |
Target: 5'- gCGcCGCAGCaaagccACCGGCgguGGCGUGACg -3' miRNA: 3'- gGC-GCGUCGaau---UGGCCG---UCGCACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 5910 | 0.66 | 0.495655 |
Target: 5'- cCCGCGCcagGGCggcacGCCGGuCAGUGagggugaaucaccUGACg -3' miRNA: 3'- -GGCGCG---UCGaau--UGGCC-GUCGC-------------ACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 21612 | 0.66 | 0.486078 |
Target: 5'- aCCGUGgAGCUUAcACCGGCguaacGGCaaaGUGGu -3' miRNA: 3'- -GGCGCgUCGAAU-UGGCCG-----UCG---CACUg -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 18372 | 0.66 | 0.475541 |
Target: 5'- aCGCcaGCAGCgggAACUGGCuGCuGUGGg -3' miRNA: 3'- gGCG--CGUCGaa-UUGGCCGuCG-CACUg -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 7630 | 0.67 | 0.434594 |
Target: 5'- uCUGUGUGGUU--GCCGGUGGUGUcGGCg -3' miRNA: 3'- -GGCGCGUCGAauUGGCCGUCGCA-CUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 6343 | 0.67 | 0.424679 |
Target: 5'- aCCGCGUcGCUUuaugagcguuACC-GCGGCGUGGa -3' miRNA: 3'- -GGCGCGuCGAAu---------UGGcCGUCGCACUg -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 5647 | 0.67 | 0.424679 |
Target: 5'- gCGCGCaaAGCUgcacaaaaucACCGGUcuguuucuGCGUGACg -3' miRNA: 3'- gGCGCG--UCGAau--------UGGCCGu-------CGCACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 11883 | 0.68 | 0.395764 |
Target: 5'- gCCgGUGCAGUaaAGCUGGCAGCGg--- -3' miRNA: 3'- -GG-CGCGUCGaaUUGGCCGUCGCacug -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 1249 | 0.68 | 0.368151 |
Target: 5'- -aGCGguGUgcugauACCGGCGGUgaGUGGCu -3' miRNA: 3'- ggCGCguCGaau---UGGCCGUCG--CACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 26534 | 0.69 | 0.35049 |
Target: 5'- aCGCGUcguuGCUguuuCUGGCagAGCGUGACc -3' miRNA: 3'- gGCGCGu---CGAauu-GGCCG--UCGCACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 28360 | 0.7 | 0.271401 |
Target: 5'- gCCGCaGCgaugggAGCUUGaggcGCUGGC-GCGUGGCg -3' miRNA: 3'- -GGCG-CG------UCGAAU----UGGCCGuCGCACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 5826 | 0.72 | 0.201675 |
Target: 5'- cCCGCGCccuGCgUAAgacCCGG-AGCGUGACa -3' miRNA: 3'- -GGCGCGu--CGaAUU---GGCCgUCGCACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 18463 | 1.12 | 0.000219 |
Target: 5'- aCCGCGCAGCUUAACCGGCAGCGUGACg -3' miRNA: 3'- -GGCGCGUCGAAUUGGCCGUCGCACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 5188 | 0.67 | 0.414901 |
Target: 5'- cUCG-GCAGCgaUGACCGcGCAGCGgGAa -3' miRNA: 3'- -GGCgCGUCGa-AUUGGC-CGUCGCaCUg -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 17732 | 0.82 | 0.040199 |
Target: 5'- aCCGCGCAGCguc-CCGGCuuggguuucaucAGCGUGACc -3' miRNA: 3'- -GGCGCGUCGaauuGGCCG------------UCGCACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 17173 | 0.74 | 0.159868 |
Target: 5'- aCCGCGaUAGUUUucgAGCUuacgggucaguuuuGGCAGCGUGACg -3' miRNA: 3'- -GGCGC-GUCGAA---UUGG--------------CCGUCGCACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 11939 | 0.73 | 0.180452 |
Target: 5'- gCCGCuGcCAGCUUuacugcACCGGCAGUgGUGAUa -3' miRNA: 3'- -GGCG-C-GUCGAAu-----UGGCCGUCG-CACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 5636 | 0.7 | 0.293514 |
Target: 5'- uCCGUcaGCAgGCgggUAuccacagacucACCGGCAGCGUGGg -3' miRNA: 3'- -GGCG--CGU-CGa--AU-----------UGGCCGUCGCACUg -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 9445 | 0.7 | 0.309021 |
Target: 5'- gCCGC-CGGgUcauACCGGCGGCaauGUGACg -3' miRNA: 3'- -GGCGcGUCgAau-UGGCCGUCG---CACUG- -5' |
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22442 | 3' | -56.2 | NC_005056.1 | + | 20576 | 0.69 | 0.359245 |
Target: 5'- uUCGCGCuGCauggucugGugUGGCAGCGUcuGGCg -3' miRNA: 3'- -GGCGCGuCGaa------UugGCCGUCGCA--CUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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