Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22476 | 3' | -40.9 | NC_005066.1 | + | 67528 | 0.66 | 1 |
Target: 5'- ----cGGUUacUACCGGAGGCGuauUAGUu -3' miRNA: 3'- aguaaCUAAa-AUGGCUUUCGCu--AUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 148947 | 0.66 | 1 |
Target: 5'- ---gUGAUuaucuuucUUUGCUGAAAggugauuauaucauuGCGAUGGCa -3' miRNA: 3'- aguaACUA--------AAAUGGCUUU---------------CGCUAUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 64769 | 0.66 | 1 |
Target: 5'- gCAUaUGAaucaGCCGAAcAGCGAUAuGCa -3' miRNA: 3'- aGUA-ACUaaaaUGGCUU-UCGCUAU-CG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 45645 | 0.66 | 1 |
Target: 5'- ----------cACCGccAGCGAUAGCa -3' miRNA: 3'- aguaacuaaaaUGGCuuUCGCUAUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 158477 | 0.66 | 1 |
Target: 5'- ----------cACCGAAAGCaGUAGCa -3' miRNA: 3'- aguaacuaaaaUGGCUUUCGcUAUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 116864 | 0.67 | 1 |
Target: 5'- cUCAUgaaaGAgcacugUUUGCCGAuGGUG-UAGCg -3' miRNA: 3'- -AGUAa---CUa-----AAAUGGCUuUCGCuAUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 86131 | 0.68 | 1 |
Target: 5'- aUCAUUGAUaUUACUGAauuuccuuauAAGCaGGUuGCa -3' miRNA: 3'- -AGUAACUAaAAUGGCU----------UUCG-CUAuCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 104630 | 0.68 | 1 |
Target: 5'- --uUUGAaauucGCCGAAGGUGAaAGCa -3' miRNA: 3'- aguAACUaaaa-UGGCUUUCGCUaUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 147816 | 0.69 | 1 |
Target: 5'- aUCGUUGGUggUACUGGAacAGCgcaaguuguuGAUAGUg -3' miRNA: 3'- -AGUAACUAaaAUGGCUU--UCG----------CUAUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 23319 | 0.69 | 1 |
Target: 5'- -uGUUGAUUugUUGCUGAAcGUGAuUAGCu -3' miRNA: 3'- agUAACUAA--AAUGGCUUuCGCU-AUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 146561 | 0.7 | 1 |
Target: 5'- cUCGcUGGUaUUGCUGAAauuguucaggcuGGCGGUAGUg -3' miRNA: 3'- -AGUaACUAaAAUGGCUU------------UCGCUAUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 94073 | 0.7 | 1 |
Target: 5'- uUCGgcGAUggaaguagccaUACCGAAAGCGAUuucAGCc -3' miRNA: 3'- -AGUaaCUAaa---------AUGGCUUUCGCUA---UCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 12105 | 0.68 | 1 |
Target: 5'- ---aUGAUUacACCGGAAGauGUAGCa -3' miRNA: 3'- aguaACUAAaaUGGCUUUCgcUAUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 122538 | 0.67 | 1 |
Target: 5'- aCggUGAUgugUGgUGGGcuAGCGAUAGCg -3' miRNA: 3'- aGuaACUAaa-AUgGCUU--UCGCUAUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 74975 | 0.68 | 1 |
Target: 5'- gUAUUGAUUUUcCCGAAAGgugGAUuGCu -3' miRNA: 3'- aGUAACUAAAAuGGCUUUCg--CUAuCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 156824 | 0.67 | 1 |
Target: 5'- aUCGcUGGgugUACCGGAagaaguauuAGCGAaaUGGCa -3' miRNA: 3'- -AGUaACUaaaAUGGCUU---------UCGCU--AUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 117008 | 0.67 | 1 |
Target: 5'- cUCAUUGAUa--ACCuGAuuGCGGcUGGCu -3' miRNA: 3'- -AGUAACUAaaaUGG-CUuuCGCU-AUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 42579 | 0.67 | 1 |
Target: 5'- aUCGcgGGgaa-ACCGGAAGCGA-AGCc -3' miRNA: 3'- -AGUaaCUaaaaUGGCUUUCGCUaUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 135667 | 0.66 | 1 |
Target: 5'- cUCGUguuaaa-GCCGAAgaccuguuuuuAGCGGUGGCa -3' miRNA: 3'- -AGUAacuaaaaUGGCUU-----------UCGCUAUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 148447 | 0.66 | 1 |
Target: 5'- ----cGAUgUUACCGAuugcugGAGCGGUuGCc -3' miRNA: 3'- aguaaCUAaAAUGGCU------UUCGCUAuCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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