Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22477 | 3' | -55.8 | NC_005066.1 | + | 145457 | 0.66 | 0.918105 |
Target: 5'- cCCUCUGCUGGUuuaguUGGcAUagagaaaGUCAAGUUUg -3' miRNA: 3'- -GGGGGCGACCG-----ACC-UAg------CAGUUCAAA- -5' |
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22477 | 3' | -55.8 | NC_005066.1 | + | 62331 | 0.66 | 0.906207 |
Target: 5'- gCCCUGCaauucaGGCggcGGAUCGUCAgcgAGUg- -3' miRNA: 3'- gGGGGCGa-----CCGa--CCUAGCAGU---UCAaa -5' |
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22477 | 3' | -55.8 | NC_005066.1 | + | 87031 | 0.7 | 0.702539 |
Target: 5'- aCCgCaCUGGCUGGuaaCGUCGAGUUUg -3' miRNA: 3'- gGGgGcGACCGACCua-GCAGUUCAAA- -5' |
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22477 | 3' | -55.8 | NC_005066.1 | + | 150251 | 1.06 | 0.004593 |
Target: 5'- uCCCCCGCUGGCUGGAUCGUCAAGUUUc -3' miRNA: 3'- -GGGGGCGACCGACCUAGCAGUUCAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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