Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22482 | 3' | -41.8 | NC_005066.1 | + | 152510 | 0.7 | 0.999998 |
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Target: 5'- uUCAACACC-GAAAAC---AAAGCCGg -3' miRNA: 3'- cAGUUGUGGaUUUUUGcuuUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 51859 | 0.7 | 0.999996 |
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Target: 5'- uUCGACuuCCUGAAAAUGGAAGA-CCGa -3' miRNA: 3'- cAGUUGu-GGAUUUUUGCUUUUUcGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 147193 | 0.7 | 0.999994 |
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Target: 5'- aUCAucACGCCUAAAAagcugcaagGCaAAAAAGCCAc -3' miRNA: 3'- cAGU--UGUGGAUUUU---------UGcUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 132148 | 0.7 | 0.999994 |
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Target: 5'- uGUCAACACCcAAuAACaAAAAAGUCGc -3' miRNA: 3'- -CAGUUGUGGaUUuUUGcUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 33115 | 0.71 | 0.999988 |
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Target: 5'- cUCAAU-CCUAAGAACGggGAA-CCAu -3' miRNA: 3'- cAGUUGuGGAUUUUUGCuuUUUcGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 53510 | 0.75 | 0.998124 |
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Target: 5'- aUCAuauCAUCauAAAACGAAAAAGCCAa -3' miRNA: 3'- cAGUu--GUGGauUUUUGCUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 22042 | 0.76 | 0.997728 |
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Target: 5'- --aAAUGCCgGAAAGCGAGAAAGCUAa -3' miRNA: 3'- cagUUGUGGaUUUUUGCUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 73940 | 0.86 | 0.809203 |
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Target: 5'- uGUCAACACC-AAAAAUaGAAAAAGCCAa -3' miRNA: 3'- -CAGUUGUGGaUUUUUG-CUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 34442 | 0.95 | 0.399689 |
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Target: 5'- uGUCAACACCccAAAACGAAAAAGCCGa -3' miRNA: 3'- -CAGUUGUGGauUUUUGCUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 47680 | 1.11 | 0.063886 |
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Target: 5'- uGUCAACACCUAAAAACGAAAAAGCCAc -3' miRNA: 3'- -CAGUUGUGGAUUUUUGCUUUUUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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