Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22482 | 3' | -41.8 | NC_005066.1 | + | 113452 | 0.67 | 1 |
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Target: 5'- gGUCaAACGCUUAAaagaaaucauGAGCGAGGAAGUa- -3' miRNA: 3'- -CAG-UUGUGGAUU----------UUUGCUUUUUCGgu -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 119056 | 0.67 | 1 |
|
Target: 5'- uUCAACACUUAuu-GCGAAGAuGUUAa -3' miRNA: 3'- cAGUUGUGGAUuuuUGCUUUUuCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 121681 | 0.66 | 1 |
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Target: 5'- uUCuACGCCUGAuu-CGAGAAcaauAGCUAg -3' miRNA: 3'- cAGuUGUGGAUUuuuGCUUUU----UCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 121957 | 0.66 | 1 |
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Target: 5'- uUCAGCGCCaacacgcuuAACGAAAAGGUUu -3' miRNA: 3'- cAGUUGUGGauuu-----UUGCUUUUUCGGu -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 124332 | 0.67 | 1 |
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Target: 5'- cGUCAuaACCUGAu--UGGAAAAGUCAu -3' miRNA: 3'- -CAGUugUGGAUUuuuGCUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 132148 | 0.7 | 0.999994 |
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Target: 5'- uGUCAACACCcAAuAACaAAAAAGUCGc -3' miRNA: 3'- -CAGUUGUGGaUUuUUGcUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 142974 | 0.66 | 1 |
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Target: 5'- uUCAAUGCUUAAugaacauGCGGuAAAAGCCGc -3' miRNA: 3'- cAGUUGUGGAUUuu-----UGCU-UUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 147193 | 0.7 | 0.999994 |
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Target: 5'- aUCAucACGCCUAAAAagcugcaagGCaAAAAAGCCAc -3' miRNA: 3'- cAGU--UGUGGAUUUU---------UGcUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 152510 | 0.7 | 0.999998 |
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Target: 5'- uUCAACACC-GAAAAC---AAAGCCGg -3' miRNA: 3'- cAGUUGUGGaUUUUUGcuuUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 152839 | 0.66 | 1 |
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Target: 5'- -aCAGCACCcu-GAACGGuuucauGCCAa -3' miRNA: 3'- caGUUGUGGauuUUUGCUuuuu--CGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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