Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22482 | 3' | -41.8 | NC_005066.1 | + | 92170 | 0.68 | 1 |
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Target: 5'- cGUCAgacGCACCaGAAAGCGAuAAAaCCGa -3' miRNA: 3'- -CAGU---UGUGGaUUUUUGCUuUUUcGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 45272 | 0.69 | 0.999999 |
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Target: 5'- uGUCAACAaCUAAAAACaaAAAGGGCUAu -3' miRNA: 3'- -CAGUUGUgGAUUUUUGc-UUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 11353 | 0.69 | 0.999999 |
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Target: 5'- -aCAACACCgacaauAGCGGAcagcAAGCCAa -3' miRNA: 3'- caGUUGUGGauuu--UUGCUUu---UUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 152510 | 0.7 | 0.999998 |
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Target: 5'- uUCAACACC-GAAAAC---AAAGCCGg -3' miRNA: 3'- cAGUUGUGGaUUUUUGcuuUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 147193 | 0.7 | 0.999994 |
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Target: 5'- aUCAucACGCCUAAAAagcugcaagGCaAAAAAGCCAc -3' miRNA: 3'- cAGU--UGUGGAUUUU---------UGcUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 132148 | 0.7 | 0.999994 |
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Target: 5'- uGUCAACACCcAAuAACaAAAAAGUCGc -3' miRNA: 3'- -CAGUUGUGGaUUuUUGcUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 53510 | 0.75 | 0.998124 |
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Target: 5'- aUCAuauCAUCauAAAACGAAAAAGCCAa -3' miRNA: 3'- cAGUu--GUGGauUUUUGCUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 34442 | 0.95 | 0.399689 |
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Target: 5'- uGUCAACACCccAAAACGAAAAAGCCGa -3' miRNA: 3'- -CAGUUGUGGauUUUUGCUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 47680 | 1.11 | 0.063886 |
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Target: 5'- uGUCAACACCUAAAAACGAAAAAGCCAc -3' miRNA: 3'- -CAGUUGUGGAUUUUUGCUUUUUCGGU- -5' |
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| 22482 | 3' | -41.8 | NC_005066.1 | + | 55397 | 0.66 | 1 |
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Target: 5'- uGUCAACAgacaUAGGAACaguAAAGGCCAu -3' miRNA: 3'- -CAGUUGUgg--AUUUUUGcu-UUUUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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