Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
225 | 3' | -53.6 | AC_000008.1 | + | 27784 | 0.66 | 0.646462 |
Target: 5'- aCAGCccguGCACCgCUGCCAGuacuGCGcACg -3' miRNA: 3'- aGUCGu---CGUGGaGGCGGUCuu--UGU-UG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 19693 | 0.66 | 0.646462 |
Target: 5'- gCGGCAGUGCCgggUCGgCGGcgguGGCGACg -3' miRNA: 3'- aGUCGUCGUGGa--GGCgGUCu---UUGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 24338 | 0.66 | 0.634807 |
Target: 5'- aUCuGCAGCGCCagUgCGCCAuuaucuGCGACg -3' miRNA: 3'- -AGuCGUCGUGG--AgGCGGUcuu---UGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 966 | 0.66 | 0.631309 |
Target: 5'- -gAGCAGCACCUugcauuuuauaucgCCGCCcau--CAACa -3' miRNA: 3'- agUCGUCGUGGA--------------GGCGGucuuuGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 30490 | 0.66 | 0.623149 |
Target: 5'- cCGGCAGCGCUUCaGCaccagCAGGAcCAGCc -3' miRNA: 3'- aGUCGUCGUGGAGgCG-----GUCUUuGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 33303 | 0.66 | 0.6115 |
Target: 5'- gCAGCauaaGGCGCCuugUCCuCCGGgcACAGCa -3' miRNA: 3'- aGUCG----UCGUGG---AGGcGGUCuuUGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 22279 | 0.67 | 0.588273 |
Target: 5'- gCAGCGcCGCCUCUGCCugcucgcgcuGuuGCAACu -3' miRNA: 3'- aGUCGUcGUGGAGGCGGu---------CuuUGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 26701 | 0.67 | 0.588273 |
Target: 5'- gCGGCAGCGgCagCGgCAGcAACAGCa -3' miRNA: 3'- aGUCGUCGUgGagGCgGUCuUUGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 26493 | 0.67 | 0.576717 |
Target: 5'- -aAGCAGcCGCCgCCGUUAGcccaaGAGCAACa -3' miRNA: 3'- agUCGUC-GUGGaGGCGGUC-----UUUGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 18076 | 0.67 | 0.576717 |
Target: 5'- gCGGUGGCGCCUUCaGCUGGGgcucgcuguggAGCGGCa -3' miRNA: 3'- aGUCGUCGUGGAGG-CGGUCU-----------UUGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 34801 | 0.68 | 0.519885 |
Target: 5'- --cGCGGcCACUUccCCGCCAGGAACcauGACa -3' miRNA: 3'- aguCGUC-GUGGA--GGCGGUCUUUG---UUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 17194 | 0.68 | 0.519885 |
Target: 5'- cCAGUAGCACCaguauugccaCCGCCAcAGAgGGCa -3' miRNA: 3'- aGUCGUCGUGGa---------GGCGGUcUUUgUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 8771 | 0.68 | 0.508769 |
Target: 5'- aCAGguGCuggcGCCggguguggCCGCUGGAGAUGACg -3' miRNA: 3'- aGUCguCG----UGGa-------GGCGGUCUUUGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 24612 | 0.68 | 0.497755 |
Target: 5'- uUCAGCuGCACCcuuggguaCCGCUcaGGAGAgGGCg -3' miRNA: 3'- -AGUCGuCGUGGa-------GGCGG--UCUUUgUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 31664 | 0.69 | 0.486848 |
Target: 5'- gCAGUGGUgcuACCUCCaccCCAGAGACGGg -3' miRNA: 3'- aGUCGUCG---UGGAGGc--GGUCUUUGUUg -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 30465 | 0.69 | 0.465385 |
Target: 5'- -gAGCAGCGCCU--GCUAGAAagacgcaggGCAGCg -3' miRNA: 3'- agUCGUCGUGGAggCGGUCUU---------UGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 23552 | 0.69 | 0.465385 |
Target: 5'- cUCuGCAGCGCCgcccgcaCCGCCGGGucCGuuGCg -3' miRNA: 3'- -AGuCGUCGUGGa------GGCGGUCUuuGU--UG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 18611 | 0.69 | 0.464324 |
Target: 5'- gCAGCGaccgccuGCACCgcggcaUCCGCCAccgcuGAGGCAACc -3' miRNA: 3'- aGUCGU-------CGUGG------AGGCGGU-----CUUUGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 14266 | 0.69 | 0.434143 |
Target: 5'- cUCcGCGGUACCUgCgGCCuaccggggggAGAAACAGCa -3' miRNA: 3'- -AGuCGUCGUGGA-GgCGG----------UCUUUGUUG- -5' |
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225 | 3' | -53.6 | AC_000008.1 | + | 4319 | 0.7 | 0.414004 |
Target: 5'- uUCAGUAGCAagCUgaUUGCCAGggGCAGg -3' miRNA: 3'- -AGUCGUCGUg-GA--GGCGGUCuuUGUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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