Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2250 | 5' | -50.6 | NC_001405.1 | + | 15066 | 0.78 | 0.240213 |
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Target: 5'- --aACAACAGUGGCAGCGgcGCGGAa- -3' miRNA: 3'- caaUGUUGUCGCCGUUGCa-CGUCUgg -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 26285 | 0.78 | 0.239545 |
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Target: 5'- --gGCGGCGGCGGCAGC-UGCAGcuucuuuuuggguGCCa -3' miRNA: 3'- caaUGUUGUCGCCGUUGcACGUC-------------UGG- -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 17125 | 0.79 | 0.191518 |
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Target: 5'- -gUGCGGCcaaucaagcaGGUGGCAccgggacuggGCGUGCAGACCg -3' miRNA: 3'- caAUGUUG----------UCGCCGU----------UGCACGUCUGG- -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 26741 | 0.8 | 0.170574 |
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Target: 5'- --gGCGGCAGCGGCAGCG-GCAG-Ca -3' miRNA: 3'- caaUGUUGUCGCCGUUGCaCGUCuGg -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 16225 | 0.81 | 0.156235 |
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Target: 5'- --gGCGGCGGUGGCGACGUGCuacgcGCCu -3' miRNA: 3'- caaUGUUGUCGCCGUUGCACGuc---UGG- -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 12605 | 1.14 | 0.000755 |
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Target: 5'- cGUUACAACAGCGGCAACGUGCAGACCa -3' miRNA: 3'- -CAAUGUUGUCGCCGUUGCACGUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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