Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2253 | 5' | -60.4 | NC_001405.1 | + | 13897 | 0.67 | 0.258306 |
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Target: 5'- -gGCCcGCGCcCGC-CCACCCGUCg- -3' miRNA: 3'- aaUGGuCGUGaGCGuGGUGGGCGGgc -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 18565 | 0.67 | 0.271963 |
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Target: 5'- cUGCCAGgGC-CGUccGCCguuguuguaACCCGCCCu -3' miRNA: 3'- aAUGGUCgUGaGCG--UGG---------UGGGCGGGc -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 24383 | 0.67 | 0.279007 |
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Target: 5'- -cGCCAuGCcUUUGguCCcCCCGCCCGa -3' miRNA: 3'- aaUGGU-CGuGAGCguGGuGGGCGGGC- -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 3131 | 0.66 | 0.301008 |
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Target: 5'- -aGCCAGCcacuCUCGCAagGCCUGgCCa -3' miRNA: 3'- aaUGGUCGu---GAGCGUggUGGGCgGGc -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 25790 | 0.66 | 0.316405 |
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Target: 5'- -aACCuauGCACccCGCACCGCUC-CCUGg -3' miRNA: 3'- aaUGGu--CGUGa-GCGUGGUGGGcGGGC- -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 26900 | 0.66 | 0.324323 |
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Target: 5'- -gACgCAGCGCUCcuccuccuGCugCugCCGCCg- -3' miRNA: 3'- aaUG-GUCGUGAG--------CGugGugGGCGGgc -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 6629 | 0.66 | 0.324323 |
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Target: 5'- cUACUucgACgCGCGCCugCUGCCCGg -3' miRNA: 3'- aAUGGucgUGaGCGUGGugGGCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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