Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2253 | 5' | -60.4 | NC_001405.1 | + | 22782 | 0.7 | 0.168398 |
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Target: 5'- -gGCCAGCG--UGCACCACCCgGCgCUGa -3' miRNA: 3'- aaUGGUCGUgaGCGUGGUGGG-CG-GGC- -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 23904 | 0.71 | 0.142615 |
|
Target: 5'- -cGCUGGUGC-CGCGCaCACCCaGCCCGc -3' miRNA: 3'- aaUGGUCGUGaGCGUG-GUGGG-CGGGC- -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 24383 | 0.67 | 0.279007 |
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Target: 5'- -cGCCAuGCcUUUGguCCcCCCGCCCGa -3' miRNA: 3'- aaUGGU-CGuGAGCguGGuGGGCGGGC- -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 25790 | 0.66 | 0.316405 |
|
Target: 5'- -aACCuauGCACccCGCACCGCUC-CCUGg -3' miRNA: 3'- aaUGGu--CGUGa-GCGUGGUGGGcGGGC- -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 26900 | 0.66 | 0.324323 |
|
Target: 5'- -gACgCAGCGCUCcuccuccuGCugCugCCGCCg- -3' miRNA: 3'- aaUG-GUCGUGAG--------CGugGugGGCGGgc -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 27697 | 0.67 | 0.25169 |
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Target: 5'- -gGCCAGCGC-CGCcgaucugaaAUguCCCGUCCGg -3' miRNA: 3'- aaUGGUCGUGaGCG---------UGguGGGCGGGC- -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 33280 | 0.69 | 0.192996 |
|
Target: 5'- --cUCAGCgaugAUUCGCACCGCCCGCa-- -3' miRNA: 3'- aauGGUCG----UGAGCGUGGUGGGCGggc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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