Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22534 | 3' | -46 | NC_005083.1 | + | 118511 | 0.7 | 0.999537 |
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Target: 5'- --aCUAGcaGACAACGCAACACuuACu -3' miRNA: 3'- uagGGUCuaUUGUUGCGUUGUGuuUG- -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 41125 | 0.69 | 0.999823 |
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Target: 5'- -aCCCAGAaauGCAACGUGAagucuGCAAACu -3' miRNA: 3'- uaGGGUCUau-UGUUGCGUUg----UGUUUG- -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 233407 | 0.76 | 0.96873 |
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Target: 5'- cAUCCCAaaacgAGCGACGCcACGCAAGCc -3' miRNA: 3'- -UAGGGUcua--UUGUUGCGuUGUGUUUG- -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 35528 | 0.68 | 0.99994 |
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Target: 5'- cUUCCAGAUAGCAcCGUGACcACGuuCg -3' miRNA: 3'- uAGGGUCUAUUGUuGCGUUG-UGUuuG- -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 26547 | 0.68 | 0.99994 |
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Target: 5'- -gCCUAGugaaAGCAACGCGACAUAu-- -3' miRNA: 3'- uaGGGUCua--UUGUUGCGUUGUGUuug -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 66088 | 0.66 | 0.999995 |
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Target: 5'- uAUCUCAGc--AUAACGCAGCaacugACGAACg -3' miRNA: 3'- -UAGGGUCuauUGUUGCGUUG-----UGUUUG- -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 73278 | 0.66 | 0.999998 |
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Target: 5'- -cUUCAGAUAGaucACGCGACGCGAuuGCu -3' miRNA: 3'- uaGGGUCUAUUgu-UGCGUUGUGUU--UG- -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 184665 | 1.1 | 0.032395 |
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Target: 5'- cAUCCCAGAUAACAACGCAACACAAACg -3' miRNA: 3'- -UAGGGUCUAUUGUUGCGUUGUGUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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