Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22534 | 3' | -46 | NC_005083.1 | + | 215345 | 0.68 | 0.999955 |
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Target: 5'- -gUCCGGGUGcaGCGGCaGCGGCAgAAACu -3' miRNA: 3'- uaGGGUCUAU--UGUUG-CGUUGUgUUUG- -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 167640 | 0.67 | 0.999982 |
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Target: 5'- aAUCCgCAGcacACGACGCAGCA-AAGCa -3' miRNA: 3'- -UAGG-GUCuauUGUUGCGUUGUgUUUG- -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 25022 | 0.67 | 0.999991 |
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Target: 5'- aAUCCC-GAUGAUuaAGCGUAAgCACAAAa -3' miRNA: 3'- -UAGGGuCUAUUG--UUGCGUU-GUGUUUg -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 200626 | 0.66 | 0.999994 |
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Target: 5'- --gCCAGAga--AGCGCGACGCAGu- -3' miRNA: 3'- uagGGUCUauugUUGCGUUGUGUUug -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 66088 | 0.66 | 0.999995 |
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Target: 5'- uAUCUCAGc--AUAACGCAGCaacugACGAACg -3' miRNA: 3'- -UAGGGUCuauUGUUGCGUUG-----UGUUUG- -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 45739 | 0.66 | 0.999997 |
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Target: 5'- uUUgUAGGU--CGugGCAACGCGAACa -3' miRNA: 3'- uAGgGUCUAuuGUugCGUUGUGUUUG- -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 29412 | 0.66 | 0.999998 |
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Target: 5'- uUCCCAGAUGcuguCGA-GCAcguACACAAAa -3' miRNA: 3'- uAGGGUCUAUu---GUUgCGU---UGUGUUUg -5' |
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| 22534 | 3' | -46 | NC_005083.1 | + | 73278 | 0.66 | 0.999998 |
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Target: 5'- -cUUCAGAUAGaucACGCGACGCGAuuGCu -3' miRNA: 3'- uaGGGUCUAUUgu-UGCGUUGUGUU--UG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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