Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22555 | 3' | -57.8 | NC_005091.1 | + | 28078 | 0.66 | 0.653177 |
Target: 5'- aCCUaCGaUCAGgu-CGUgUCGGCCGGCGa -3' miRNA: 3'- -GGA-GC-GGUCaagGCGaAGCCGGCUGC- -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 12195 | 0.66 | 0.653177 |
Target: 5'- gCUCGCCGugccGUUCCGCaUCaucaagcaGGcCCGGCu -3' miRNA: 3'- gGAGCGGU----CAAGGCGaAG--------CC-GGCUGc -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 50288 | 0.66 | 0.631919 |
Target: 5'- gUCUCaGCCAucgUCgGCUcgCGGCCGAUc -3' miRNA: 3'- -GGAG-CGGUca-AGgCGAa-GCCGGCUGc -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 50385 | 0.66 | 0.621286 |
Target: 5'- aCCU-GCCGGgugaUCgGCcgCGaGCCGACGa -3' miRNA: 3'- -GGAgCGGUCa---AGgCGaaGC-CGGCUGC- -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 31707 | 0.66 | 0.610661 |
Target: 5'- gCCgCGCCGG--CCGcCUUCGGCUG-CGc -3' miRNA: 3'- -GGaGCGGUCaaGGC-GAAGCCGGCuGC- -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 25807 | 0.66 | 0.610661 |
Target: 5'- --gCGCCGG--CUGCcUUGGCCGACa -3' miRNA: 3'- ggaGCGGUCaaGGCGaAGCCGGCUGc -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 9283 | 0.66 | 0.610661 |
Target: 5'- gCUCGCCGcuggCCGCcgacCGGCUGGCc -3' miRNA: 3'- gGAGCGGUcaa-GGCGaa--GCCGGCUGc -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 43164 | 0.67 | 0.589472 |
Target: 5'- aCUCGCgacgagCAGUucuUCUGCUUUcgcggGGCUGACGa -3' miRNA: 3'- gGAGCG------GUCA---AGGCGAAG-----CCGGCUGC- -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 49602 | 0.67 | 0.589472 |
Target: 5'- -aUUGUCGGUUCCGU---GGCUGGCGc -3' miRNA: 3'- ggAGCGGUCAAGGCGaagCCGGCUGC- -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 22835 | 0.67 | 0.578924 |
Target: 5'- aCgCGCCuGaacgCCGCgcugaCGGCCGGCGg -3' miRNA: 3'- gGaGCGGuCaa--GGCGaa---GCCGGCUGC- -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 7225 | 0.67 | 0.557958 |
Target: 5'- uCCUUgGCCGGggcgUCC---UCGGCCGGCa -3' miRNA: 3'- -GGAG-CGGUCa---AGGcgaAGCCGGCUGc -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 26687 | 0.68 | 0.506624 |
Target: 5'- gCUUGCUg---CUGCggCGGCCGGCGg -3' miRNA: 3'- gGAGCGGucaaGGCGaaGCCGGCUGC- -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 14463 | 0.69 | 0.476802 |
Target: 5'- uCCUCGUCGGcugUCCGggUCaGGCgGGCa -3' miRNA: 3'- -GGAGCGGUCa--AGGCgaAG-CCGgCUGc -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 53152 | 0.69 | 0.476802 |
Target: 5'- gUCUCGCguGaUUCCGCUguagCGcGCgGGCGc -3' miRNA: 3'- -GGAGCGguC-AAGGCGAa---GC-CGgCUGC- -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 46534 | 0.69 | 0.467054 |
Target: 5'- gCUCGCCccUUCCGCUcaacgcugcUCGGCaGAUGa -3' miRNA: 3'- gGAGCGGucAAGGCGA---------AGCCGgCUGC- -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 45788 | 0.69 | 0.447875 |
Target: 5'- ---aGCCGGcgCgGCgcgCGGCCGACGc -3' miRNA: 3'- ggagCGGUCaaGgCGaa-GCCGGCUGC- -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 17838 | 0.7 | 0.375834 |
Target: 5'- --gCGCCAcggcgacaaacGUUCCGCUaUCGGCCGGg- -3' miRNA: 3'- ggaGCGGU-----------CAAGGCGA-AGCCGGCUgc -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 11013 | 0.71 | 0.359086 |
Target: 5'- gCUUCGCCGGggUCGCUUugcCGGgCGGCu -3' miRNA: 3'- -GGAGCGGUCaaGGCGAA---GCCgGCUGc -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 17964 | 0.71 | 0.342867 |
Target: 5'- gUUCGCCAGUUgUCGC--CGGCCGAUc -3' miRNA: 3'- gGAGCGGUCAA-GGCGaaGCCGGCUGc -5' |
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22555 | 3' | -57.8 | NC_005091.1 | + | 11415 | 0.71 | 0.334957 |
Target: 5'- gCCU-GCCccuGUUUCGCggcggccacgUCGGCCGACGc -3' miRNA: 3'- -GGAgCGGu--CAAGGCGa---------AGCCGGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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