Results 1 - 20 of 52 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22557 | 3' | -57.2 | NC_005091.1 | + | 56959 | 0.71 | 0.292705 |
Target: 5'- aUGCucgaauGCGACGCaGAGCGGgCcgGCCg -3' miRNA: 3'- aACGucu---CGCUGUG-CUCGCC-GuaCGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 55844 | 0.67 | 0.528217 |
Target: 5'- -cGCAGccggucagcaacuGGCG-CACGAGCuGCAcgGCCu -3' miRNA: 3'- aaCGUC-------------UCGCuGUGCUCGcCGUa-CGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 55598 | 0.72 | 0.257861 |
Target: 5'- gUGCAGgcGGCGAUcgGCGAagugauGCGGCAaGCCg -3' miRNA: 3'- aACGUC--UCGCUG--UGCU------CGCCGUaCGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 55399 | 0.73 | 0.226484 |
Target: 5'- -aGCGcGAGC-ACGCGGGCGGCAaagcUGUCg -3' miRNA: 3'- aaCGU-CUCGcUGUGCUCGCCGU----ACGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 52295 | 0.66 | 0.594056 |
Target: 5'- gUUGCGGAuCGAUuCGAcaGCGGCcaGUGCUg -3' miRNA: 3'- -AACGUCUcGCUGuGCU--CGCCG--UACGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 52228 | 0.66 | 0.594056 |
Target: 5'- gUGCGGGGCGGCuuugAC-AGCGGCuugaaccuuUGCg -3' miRNA: 3'- aACGUCUCGCUG----UGcUCGCCGu--------ACGg -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 51600 | 0.75 | 0.177687 |
Target: 5'- -cGCAGAagcGCGGCACGGGCgcgauucGGCAugauaUGCCg -3' miRNA: 3'- aaCGUCU---CGCUGUGCUCG-------CCGU-----ACGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 51561 | 0.68 | 0.437698 |
Target: 5'- gUGCAu-GCGAUcCGcGCGGCAUcGCCa -3' miRNA: 3'- aACGUcuCGCUGuGCuCGCCGUA-CGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 49867 | 0.66 | 0.572247 |
Target: 5'- -gGCAGaAGcCGAUcgagcCGAGCGugcuGCAUGCCa -3' miRNA: 3'- aaCGUC-UC-GCUGu----GCUCGC----CGUACGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 49484 | 0.68 | 0.477357 |
Target: 5'- -aGCAGGccaauuGCGAacacgACGAGCGGCAgGCa -3' miRNA: 3'- aaCGUCU------CGCUg----UGCUCGCCGUaCGg -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 48763 | 0.67 | 0.539919 |
Target: 5'- -gGCcGuGCGcacGCAUGA-CGGCAUGCCu -3' miRNA: 3'- aaCGuCuCGC---UGUGCUcGCCGUACGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 48720 | 0.72 | 0.251313 |
Target: 5'- cUGCAcGAGCGACACGGagauaCGGcCAUGCg -3' miRNA: 3'- aACGU-CUCGCUGUGCUc----GCC-GUACGg -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 47659 | 0.71 | 0.307638 |
Target: 5'- -aGCGGuucauGGCGAuCAUauAGCGGCGUGCCg -3' miRNA: 3'- aaCGUC-----UCGCU-GUGc-UCGCCGUACGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 47447 | 0.69 | 0.399996 |
Target: 5'- -aGUAGcGCGugucguuccGCACGAGCGGCA-GCg -3' miRNA: 3'- aaCGUCuCGC---------UGUGCUCGCCGUaCGg -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 45794 | 0.67 | 0.497842 |
Target: 5'- -aGCAGuAGcCGGCGCG-GCGcGCG-GCCg -3' miRNA: 3'- aaCGUC-UC-GCUGUGCuCGC-CGUaCGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 44887 | 0.76 | 0.155469 |
Target: 5'- -aGCAG-GCGGUACGGGCGGCGgcaaggcgGCCg -3' miRNA: 3'- aaCGUCuCGCUGUGCUCGCCGUa-------CGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 43611 | 0.68 | 0.457297 |
Target: 5'- uUUGuCGGA-CGGCGUGAGCGGCGcaccuuucUGCCa -3' miRNA: 3'- -AAC-GUCUcGCUGUGCUCGCCGU--------ACGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 43245 | 0.71 | 0.315321 |
Target: 5'- -aGCAGAagaacuGCucguCGCGAGUGGCAUGCg -3' miRNA: 3'- aaCGUCU------CGcu--GUGCUCGCCGUACGg -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 41429 | 0.67 | 0.497842 |
Target: 5'- cUGUAcGAGCugGAUcagcucaucaACGAucGCGGCGUGCCg -3' miRNA: 3'- aACGU-CUCG--CUG----------UGCU--CGCCGUACGG- -5' |
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22557 | 3' | -57.2 | NC_005091.1 | + | 39333 | 0.73 | 0.213169 |
Target: 5'- cUGCGuAGCGACACGcAGCGGCgcgaucacgagacaGUGCUu -3' miRNA: 3'- aACGUcUCGCUGUGC-UCGCCG--------------UACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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