miRNA: 3'- cCCUGUGCGGCcuuCGU-UGGCACGga -5'
miRNA: 3'- cCCuGUGCGGCCuuCGUu--GGCA---CGGA- -5'
miRNA: 3'- cCCUGUGCGGCCU-UCGUUGGcacGGa -5'
miRNA: 3'- ccCUGUGCGGCCU--------UCGUUGgCACGga -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22557 | 5' | -57.9 | NC_005091.1 | + | 49045 | 0.66 | 0.614301 |
|
Target: 5'- cGGGCGCGCUGcu-GCGcACCGUGUg- -3' miRNA: 3'- cCCUGUGCGGCcuuCGU-UGGCACGga -5' |
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| 22557 | 5' | -57.9 | NC_005091.1 | + | 49551 | 0.68 | 0.459195 |
|
Target: 5'- cGGcCGCGUCGGggGCGucuUCGUaccGCCUg -3' miRNA: 3'- cCCuGUGCGGCCuuCGUu--GGCA---CGGA- -5' |
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| 22557 | 5' | -57.9 | NC_005091.1 | + | 52452 | 0.7 | 0.359669 |
|
Target: 5'- aGGGCGCGCCGGGcGGCGACC---CCa -3' miRNA: 3'- cCCUGUGCGGCCU-UCGUUGGcacGGa -5' |
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| 22557 | 5' | -57.9 | NC_005091.1 | + | 53659 | 0.7 | 0.359669 |
|
Target: 5'- -cGGCGCGCCGGGguugcgcuGGCAAUgGUGCg- -3' miRNA: 3'- ccCUGUGCGGCCU--------UCGUUGgCACGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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