Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22557 | 5' | -57.9 | NC_005091.1 | + | 23419 | 0.67 | 0.560991 |
Target: 5'- cGGGACGugggGCCuuGgcGCuuCCGUGCCUg -3' miRNA: 3'- -CCCUGUg---CGGc-CuuCGuuGGCACGGA- -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 11481 | 0.66 | 0.603576 |
Target: 5'- aGGGGCAgGCCGaagcgcagcguGAcGCGGCCGacGCCc -3' miRNA: 3'- -CCCUGUgCGGC-----------CUuCGUUGGCa-CGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 33427 | 0.66 | 0.614301 |
Target: 5'- cGGACGCGaCGc-AGCAACCGgcauUGCCg -3' miRNA: 3'- cCCUGUGCgGCcuUCGUUGGC----ACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 3417 | 1.09 | 0.000623 |
Target: 5'- aGGGACACGCCGGAAGCAACCGUGCCUu -3' miRNA: 3'- -CCCUGUGCGGCCUUCGUUGGCACGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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