Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22564 | 5' | -57.6 | NC_005091.1 | + | 30623 | 0.74 | 0.206663 |
Target: 5'- cGCCGAa-GCAcUGCGUGCCUUGGagacCCg -3' miRNA: 3'- aCGGCUcaCGUuACGCGCGGAACC----GG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 34546 | 0.74 | 0.19602 |
Target: 5'- cUGCCGAGUGgGAccGCGCGgCgaaccGGCCg -3' miRNA: 3'- -ACGGCUCACgUUa-CGCGCgGaa---CCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 10891 | 1.12 | 0.00033 |
Target: 5'- cUGCCGAGUGCAAUGCGCGCCUUGGCCg -3' miRNA: 3'- -ACGGCUCACGUUACGCGCGGAACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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