Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22566 | 3' | -56.2 | NC_005091.1 | + | 22919 | 0.74 | 0.318038 |
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Target: 5'- aUGGCGgcCGAGCAGCGGCGUucgcucCGGa -3' miRNA: 3'- gGCCGCa-GCUCGUCGCUGCAcuu---GCC- -5' |
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| 22566 | 3' | -56.2 | NC_005091.1 | + | 29473 | 0.74 | 0.288909 |
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Target: 5'- uUCGGCGaacCGGGCgAGCGACGUaGGACGc -3' miRNA: 3'- -GGCCGCa--GCUCG-UCGCUGCA-CUUGCc -5' |
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| 22566 | 3' | -56.2 | NC_005091.1 | + | 6983 | 0.75 | 0.281962 |
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Target: 5'- cCCGGCGucaUCGAGCugcuGGCGGaaggcaagGUGAGCGGc -3' miRNA: 3'- -GGCCGC---AGCUCG----UCGCUg-------CACUUGCC- -5' |
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| 22566 | 3' | -56.2 | NC_005091.1 | + | 18164 | 0.76 | 0.243046 |
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Target: 5'- cCCGGCGaUCGAGC-GUGAgCGUGAACc- -3' miRNA: 3'- -GGCCGC-AGCUCGuCGCU-GCACUUGcc -5' |
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| 22566 | 3' | -56.2 | NC_005091.1 | + | 16524 | 0.85 | 0.056715 |
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Target: 5'- cCCGGCGUCGAGCauGGCGACGcUGu-CGGa -3' miRNA: 3'- -GGCCGCAGCUCG--UCGCUGC-ACuuGCC- -5' |
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| 22566 | 3' | -56.2 | NC_005091.1 | + | 13596 | 1.12 | 0.000784 |
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Target: 5'- cCCGGCGUCGAGCAGCGACGUGAACGGu -3' miRNA: 3'- -GGCCGCAGCUCGUCGCUGCACUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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