Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22568 | 5' | -53.1 | NC_005091.1 | + | 44619 | 0.66 | 0.884952 |
Target: 5'- uGAUCgagGCGAgGUC-UUCGGCCUUGCc- -3' miRNA: 3'- -CUAG---CGCUgCAGcAAGUUGGAGCGcc -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 24581 | 0.66 | 0.884952 |
Target: 5'- cGAUCgggGCGGCGUCGUUgCAaggcgcgcGCCcugUCGaCGGa -3' miRNA: 3'- -CUAG---CGCUGCAGCAA-GU--------UGG---AGC-GCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 30215 | 0.66 | 0.877429 |
Target: 5'- --cCGgGGCGUC-UUCGACCUCGaCa- -3' miRNA: 3'- cuaGCgCUGCAGcAAGUUGGAGC-Gcc -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 24534 | 0.66 | 0.869657 |
Target: 5'- gGGUcCGCGAUGUCGUaCAAgC-CGcCGGa -3' miRNA: 3'- -CUA-GCGCUGCAGCAaGUUgGaGC-GCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 1749 | 0.66 | 0.869657 |
Target: 5'- cGUCGCGACGg---UCGACCUCa--- -3' miRNA: 3'- cUAGCGCUGCagcaAGUUGGAGcgcc -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 39128 | 0.66 | 0.86164 |
Target: 5'- -cUCGCGccACGucUCGUUCAAgCUCG-GGa -3' miRNA: 3'- cuAGCGC--UGC--AGCAAGUUgGAGCgCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 35513 | 0.66 | 0.853387 |
Target: 5'- gGGUCGCcACGUCGgUCAAgCguaaugagUCGUGGg -3' miRNA: 3'- -CUAGCGcUGCAGCaAGUUgG--------AGCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 25322 | 0.66 | 0.853387 |
Target: 5'- ---gGCGACGU-GaUCGGCCUCGUGc -3' miRNA: 3'- cuagCGCUGCAgCaAGUUGGAGCGCc -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 26221 | 0.67 | 0.836203 |
Target: 5'- --aCGCGGCGgcaGcUUCGGCCUgaGCGGc -3' miRNA: 3'- cuaGCGCUGCag-C-AAGUUGGAg-CGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 375 | 0.67 | 0.836203 |
Target: 5'- cGUCGaCGcCGUCGUgaaggCGGCCaaGCGGc -3' miRNA: 3'- cUAGC-GCuGCAGCAa----GUUGGagCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 57769 | 0.67 | 0.836203 |
Target: 5'- cGUCGaCGcCGUCGUgaaggCGGCCaaGCGGc -3' miRNA: 3'- cUAGC-GCuGCAGCAa----GUUGGagCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 24098 | 0.67 | 0.827289 |
Target: 5'- cGGUUGCGA--UCGUUCggUCUgCGUGGc -3' miRNA: 3'- -CUAGCGCUgcAGCAAGuuGGA-GCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 34448 | 0.67 | 0.808862 |
Target: 5'- aGGUCGCGuACGgccgGUUCG-CCgCGCGGu -3' miRNA: 3'- -CUAGCGC-UGCag--CAAGUuGGaGCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 18463 | 0.68 | 0.783826 |
Target: 5'- gGGUCGUaguccgagcggaucaGACGcagCG-UCAGCUUCGCGGu -3' miRNA: 3'- -CUAGCG---------------CUGCa--GCaAGUUGGAGCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 14291 | 0.69 | 0.739164 |
Target: 5'- cGUCaCGAUG-CGUaUCGGCUUCGCGGu -3' miRNA: 3'- cUAGcGCUGCaGCA-AGUUGGAGCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 44658 | 0.69 | 0.728692 |
Target: 5'- uGAUCGCcuucGCGUCGUccaUCGACUUuucguccuugaCGCGGa -3' miRNA: 3'- -CUAGCGc---UGCAGCA---AGUUGGA-----------GCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 43165 | 0.69 | 0.718124 |
Target: 5'- -cUCGCGACGagcaGUUCuucuGCuUUCGCGGg -3' miRNA: 3'- cuAGCGCUGCag--CAAGu---UG-GAGCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 42121 | 0.69 | 0.707473 |
Target: 5'- -uUCGCuGAUGUCGUUCuuGCCUacgaucCGUGGu -3' miRNA: 3'- cuAGCG-CUGCAGCAAGu-UGGA------GCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 51063 | 0.7 | 0.664247 |
Target: 5'- --gCGCGcCGcuUCGaUCAacGCCUCGCGGa -3' miRNA: 3'- cuaGCGCuGC--AGCaAGU--UGGAGCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 33347 | 0.7 | 0.642414 |
Target: 5'- --aCGCGACGcacacaucgcaaUCacccgGUUCAACCUgCGCGGg -3' miRNA: 3'- cuaGCGCUGC------------AG-----CAAGUUGGA-GCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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