Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22568 | 5' | -53.1 | NC_005091.1 | + | 41403 | 0.7 | 0.631478 |
Target: 5'- cGAUCGCGGCGUgcCGgUCGAUCUCGa-- -3' miRNA: 3'- -CUAGCGCUGCA--GCaAGUUGGAGCgcc -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 26113 | 0.71 | 0.609618 |
Target: 5'- uGAUCGCGcucgccacggcguACGUCGgcgugcagggcgcgaUUAACCUUGCGGc -3' miRNA: 3'- -CUAGCGC-------------UGCAGCa--------------AGUUGGAGCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 42048 | 0.73 | 0.50278 |
Target: 5'- ---gGCGACGUgcUGcUCGACCUCGCGa -3' miRNA: 3'- cuagCGCUGCA--GCaAGUUGGAGCGCc -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 50275 | 0.74 | 0.462261 |
Target: 5'- cAUUGCGGCGaUCGUcUCAGCCaucgucggcUCGCGGc -3' miRNA: 3'- cUAGCGCUGC-AGCA-AGUUGG---------AGCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 36342 | 0.75 | 0.386802 |
Target: 5'- cGUCGUGGCGgCGUUC-AUCUCGUGGc -3' miRNA: 3'- cUAGCGCUGCaGCAAGuUGGAGCGCC- -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 36802 | 0.83 | 0.127332 |
Target: 5'- uGAUgGCGACGgcgagcCGUUCGACCUCGCGu -3' miRNA: 3'- -CUAgCGCUGCa-----GCAAGUUGGAGCGCc -5' |
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22568 | 5' | -53.1 | NC_005091.1 | + | 14137 | 0.91 | 0.038321 |
Target: 5'- aGAUCG-GcACGUCGUUCAACCUCGCGGc -3' miRNA: 3'- -CUAGCgC-UGCAGCAAGUUGGAGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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