Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22568 | 5' | -53.1 | NC_005091.1 | + | 25322 | 0.66 | 0.853387 |
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Target: 5'- ---gGCGACGU-GaUCGGCCUCGUGc -3' miRNA: 3'- cuagCGCUGCAgCaAGUUGGAGCGCc -5' |
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| 22568 | 5' | -53.1 | NC_005091.1 | + | 39128 | 0.66 | 0.86164 |
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Target: 5'- -cUCGCGccACGucUCGUUCAAgCUCG-GGa -3' miRNA: 3'- cuAGCGC--UGC--AGCAAGUUgGAGCgCC- -5' |
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| 22568 | 5' | -53.1 | NC_005091.1 | + | 1749 | 0.66 | 0.869657 |
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Target: 5'- cGUCGCGACGg---UCGACCUCa--- -3' miRNA: 3'- cUAGCGCUGCagcaAGUUGGAGcgcc -5' |
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| 22568 | 5' | -53.1 | NC_005091.1 | + | 24534 | 0.66 | 0.869657 |
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Target: 5'- gGGUcCGCGAUGUCGUaCAAgC-CGcCGGa -3' miRNA: 3'- -CUA-GCGCUGCAGCAaGUUgGaGC-GCC- -5' |
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| 22568 | 5' | -53.1 | NC_005091.1 | + | 30215 | 0.66 | 0.877429 |
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Target: 5'- --cCGgGGCGUC-UUCGACCUCGaCa- -3' miRNA: 3'- cuaGCgCUGCAGcAAGUUGGAGC-Gcc -5' |
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| 22568 | 5' | -53.1 | NC_005091.1 | + | 24581 | 0.66 | 0.884952 |
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Target: 5'- cGAUCgggGCGGCGUCGUUgCAaggcgcgcGCCcugUCGaCGGa -3' miRNA: 3'- -CUAG---CGCUGCAGCAA-GU--------UGG---AGC-GCC- -5' |
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| 22568 | 5' | -53.1 | NC_005091.1 | + | 44619 | 0.66 | 0.884952 |
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Target: 5'- uGAUCgagGCGAgGUC-UUCGGCCUUGCc- -3' miRNA: 3'- -CUAG---CGCUgCAGcAAGUUGGAGCGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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