Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22570 | 3' | -55.6 | NC_005091.1 | + | 40558 | 0.67 | 0.710376 |
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Target: 5'- gCCGA-GUCgGuAUCCC-GCGcCGAGCa -3' miRNA: 3'- -GGCUcCAGgUuUAGGGcCGCaGCUCG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 40840 | 0.66 | 0.771577 |
|
Target: 5'- gCCGAgcaGGUCguAAUCCCccgcucggauGGCGUUcgugaccgugcaGAGCg -3' miRNA: 3'- -GGCU---CCAGguUUAGGG----------CCGCAG------------CUCG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 48120 | 0.66 | 0.751612 |
|
Target: 5'- aUCGaAGGUCCGAAuguUUuuGGCGUCa--- -3' miRNA: 3'- -GGC-UCCAGGUUU---AGggCCGCAGcucg -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 48603 | 0.68 | 0.657259 |
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Target: 5'- gUCGGGGUCCca--UCCGGCGagGcGCg -3' miRNA: 3'- -GGCUCCAGGuuuaGGGCCGCagCuCG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 51789 | 0.68 | 0.646529 |
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Target: 5'- uCCGcGaUCU---UCCCGGCGUcCGGGCg -3' miRNA: 3'- -GGCuCcAGGuuuAGGGCCGCA-GCUCG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 56130 | 0.74 | 0.315113 |
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Target: 5'- aCCGgcaucuugcucaggaAGGUCCGGAUUCCaGCG-CGGGCg -3' miRNA: 3'- -GGC---------------UCCAGGUUUAGGGcCGCaGCUCG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 57254 | 0.67 | 0.699858 |
|
Target: 5'- gCCGAucaUCCcgcaaCCCGGCGUCG-GCa -3' miRNA: 3'- -GGCUcc-AGGuuua-GGGCCGCAGCuCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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