Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22570 | 5' | -52.7 | NC_005091.1 | + | 53504 | 0.66 | 0.891936 |
Target: 5'- gUGUCGccauCGCCGuguuCAUCggCGAACCa -3' miRNA: 3'- gACAGCcu--GCGGUu---GUAGa-GCUUGGg -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 55798 | 0.66 | 0.891936 |
Target: 5'- aCUG-CGGcuGCGCCGACugCUCGGGCa- -3' miRNA: 3'- -GACaGCC--UGCGGUUGuaGAGCUUGgg -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 10427 | 0.66 | 0.891936 |
Target: 5'- aUGUCGGGCGCgCGACAcg-CGcaguACUCu -3' miRNA: 3'- gACAGCCUGCG-GUUGUagaGCu---UGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 41092 | 0.66 | 0.891936 |
Target: 5'- -cGUCGGccccgGCCGGCGUCUcCGuuuuCCCc -3' miRNA: 3'- gaCAGCCug---CGGUUGUAGA-GCuu--GGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 33582 | 0.66 | 0.88459 |
Target: 5'- aUG-CGGAucgaUGCCGGCcUCUCG-ACCUa -3' miRNA: 3'- gACaGCCU----GCGGUUGuAGAGCuUGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 28143 | 0.66 | 0.88459 |
Target: 5'- -aGcCGGAUgcaGCCGACAUCacCGAcacgaaGCCCa -3' miRNA: 3'- gaCaGCCUG---CGGUUGUAGa-GCU------UGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 11663 | 0.66 | 0.869122 |
Target: 5'- -cGUaCGGACaGCCGaaGCGccagCUCGAugCCc -3' miRNA: 3'- gaCA-GCCUG-CGGU--UGUa---GAGCUugGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 40998 | 0.66 | 0.869122 |
Target: 5'- --cUCGGccguACGCCGAaagcgCUCGAACCg -3' miRNA: 3'- gacAGCC----UGCGGUUgua--GAGCUUGGg -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 24621 | 0.66 | 0.852665 |
Target: 5'- -cGUCcGGCGgcuuguaCGACAUCgCGGACCCg -3' miRNA: 3'- gaCAGcCUGCg------GUUGUAGaGCUUGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 7174 | 0.66 | 0.852665 |
Target: 5'- uUGcCGGcaGCGgCGGcCGUCUCGAugCCc -3' miRNA: 3'- gACaGCC--UGCgGUU-GUAGAGCUugGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 19631 | 0.66 | 0.850114 |
Target: 5'- uCUGUCGGcgGCGCucggcccgaaagcgCAGCAaC-CGGACCCc -3' miRNA: 3'- -GACAGCC--UGCG--------------GUUGUaGaGCUUGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 7002 | 0.67 | 0.817033 |
Target: 5'- -gGUCGGAgauguucaguucCGCCAugAUCucuuucuucgUCGcGCCCg -3' miRNA: 3'- gaCAGCCU------------GCGGUugUAG----------AGCuUGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 34479 | 0.67 | 0.807612 |
Target: 5'- cCUGUCGaACccguaCCAGCAaCUCGAcacGCCCa -3' miRNA: 3'- -GACAGCcUGc----GGUUGUaGAGCU---UGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 22847 | 0.67 | 0.807612 |
Target: 5'- gCUGcUCGGcCGCCAuauucgauugcuGCAcgCUCGucGCCCa -3' miRNA: 3'- -GAC-AGCCuGCGGU------------UGUa-GAGCu-UGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 26729 | 0.68 | 0.778282 |
Target: 5'- -cGcCGGGcCGCCGACucgaUCGAccGCCCg -3' miRNA: 3'- gaCaGCCU-GCGGUUGuag-AGCU--UGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 44024 | 0.68 | 0.757949 |
Target: 5'- ---aUGGACGUgGGCGgcuUCGAACCCg -3' miRNA: 3'- gacaGCCUGCGgUUGUag-AGCUUGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 40464 | 0.69 | 0.737099 |
Target: 5'- -aGUCGuGCGCCucuuggcggGACAUgUCGAuACCCa -3' miRNA: 3'- gaCAGCcUGCGG---------UUGUAgAGCU-UGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 6146 | 0.7 | 0.694216 |
Target: 5'- -aGUCGGACGCgAacuuucugGCGUCuccgucguccUCGAacGCCCg -3' miRNA: 3'- gaCAGCCUGCGgU--------UGUAG----------AGCU--UGGG- -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 45617 | 0.7 | 0.694216 |
Target: 5'- -cGcCGGGCGUCGauaucgACAUCUCGGACg- -3' miRNA: 3'- gaCaGCCUGCGGU------UGUAGAGCUUGgg -5' |
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22570 | 5' | -52.7 | NC_005091.1 | + | 29577 | 0.7 | 0.683316 |
Target: 5'- gUGaUCGGcgcGCGCCAGCGUCcuacgUCGcucGCCCg -3' miRNA: 3'- gAC-AGCC---UGCGGUUGUAG-----AGCu--UGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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