Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22581 | 3' | -51.1 | NC_005091.1 | + | 6683 | 0.66 | 0.912608 |
Target: 5'- --cCcgGACGC-CGCGCGaGC--GAUCa -3' miRNA: 3'- caaGuaCUGCGaGCGCGC-CGaaUUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 33267 | 0.66 | 0.905857 |
Target: 5'- --aCAUGAagcaGCUCGCgGCGGCc----- -3' miRNA: 3'- caaGUACUg---CGAGCG-CGCCGaauuag -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 45785 | 0.66 | 0.898815 |
Target: 5'- -----cGGCGCggCGCGCGGCcgAcgCg -3' miRNA: 3'- caaguaCUGCGa-GCGCGCCGaaUuaG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 22766 | 0.66 | 0.891484 |
Target: 5'- cGUUCA-GGCGCgugCGCuCGGCaucGAUCa -3' miRNA: 3'- -CAAGUaCUGCGa--GCGcGCCGaa-UUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 2613 | 0.66 | 0.883872 |
Target: 5'- aGUUCGgguuGCGCaCGCGCGGCagaacgUGAUUg -3' miRNA: 3'- -CAAGUac--UGCGaGCGCGCCGa-----AUUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 31870 | 0.66 | 0.883872 |
Target: 5'- -gUCGUcGCGCUCGUcgGCGGCcUGcUCg -3' miRNA: 3'- caAGUAcUGCGAGCG--CGCCGaAUuAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 52888 | 0.66 | 0.883095 |
Target: 5'- ----uUGAUGCUgcugccgCGUGCGGCcUGAUCg -3' miRNA: 3'- caaguACUGCGA-------GCGCGCCGaAUUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 15146 | 0.67 | 0.850719 |
Target: 5'- -aUCcgGugGUUCGCauGCGGCgg-GUCu -3' miRNA: 3'- caAGuaCugCGAGCG--CGCCGaauUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 15026 | 0.67 | 0.850719 |
Target: 5'- -aUCcgGugGUUCGCauGCGGCgg-GUCu -3' miRNA: 3'- caAGuaCugCGAGCG--CGCCGaauUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 14906 | 0.67 | 0.850719 |
Target: 5'- -aUCcgGugGUUCGCauGCGGCgg-GUCu -3' miRNA: 3'- caAGuaCugCGAGCG--CGCCGaauUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 18365 | 0.68 | 0.832632 |
Target: 5'- --aCGUGACgaucuGCUCGuUGCGGCUcguGUCg -3' miRNA: 3'- caaGUACUG-----CGAGC-GCGCCGAau-UAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 47327 | 0.68 | 0.823245 |
Target: 5'- uGUUCGUGAUGa----GCGGCUUGAUUu -3' miRNA: 3'- -CAAGUACUGCgagcgCGCCGAAUUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 13886 | 0.68 | 0.813641 |
Target: 5'- -gUCAgcacGACGCUCGuUGCGGCa----- -3' miRNA: 3'- caAGUa---CUGCGAGC-GCGCCGaauuag -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 19666 | 0.68 | 0.803832 |
Target: 5'- -gUCGUGugGCUCGUGCucauGGUcgcGUCg -3' miRNA: 3'- caAGUACugCGAGCGCG----CCGaauUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 52718 | 0.68 | 0.803832 |
Target: 5'- gGUUCGggcauCGCUUGgGCGGCU--GUCg -3' miRNA: 3'- -CAAGUacu--GCGAGCgCGCCGAauUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 51797 | 0.69 | 0.773297 |
Target: 5'- cUUCccGGCGUccgggCGUGCGGCUUGcgCg -3' miRNA: 3'- cAAGuaCUGCGa----GCGCGCCGAAUuaG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 49824 | 0.69 | 0.762788 |
Target: 5'- cGUUCGUGGCgGCUUgcgcguugaGCGCGGCa----- -3' miRNA: 3'- -CAAGUACUG-CGAG---------CGCGCCGaauuag -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 4805 | 0.7 | 0.730451 |
Target: 5'- -gUCGUGgauucucuguucGCGCUCgGCGCGGCggcaacGGUCa -3' miRNA: 3'- caAGUAC------------UGCGAG-CGCGCCGaa----UUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 26142 | 0.7 | 0.708343 |
Target: 5'- -gUCGUGguaucaGCGCgUGCGCGGCgagcUGAUCg -3' miRNA: 3'- caAGUAC------UGCGaGCGCGCCGa---AUUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 20119 | 0.7 | 0.708343 |
Target: 5'- -gUCgAUGACGUUCaaGCGCGGCg--GUCu -3' miRNA: 3'- caAG-UACUGCGAG--CGCGCCGaauUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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