Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22623 | 3' | -57 | NC_005091.1 | + | 23440 | 0.65 | 0.644879 |
Target: 5'- cGCCaUGaucugcaugaugacGCUCGCcaucuGGCGCAGgaacUGCGCAg -3' miRNA: 3'- -CGG-AC--------------UGAGCGuu---CCGCGUU----GCGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 33443 | 0.66 | 0.626328 |
Target: 5'- aGCgCUcGCUCgGCGAcGGaCGCGACGCaGCAa -3' miRNA: 3'- -CG-GAcUGAG-CGUU-CC-GCGUUGCG-CGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 53146 | 0.66 | 0.61542 |
Target: 5'- cGCgCUGuCUCGCGugauuccGCuGUAGCGCGCGg -3' miRNA: 3'- -CG-GACuGAGCGUuc-----CG-CGUUGCGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 36575 | 0.66 | 0.604526 |
Target: 5'- gGCCgaGAaucccCUUGCAuggGGGCGCuuCGUGCGg -3' miRNA: 3'- -CGGa-CU-----GAGCGU---UCCGCGuuGCGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 3513 | 0.66 | 0.604526 |
Target: 5'- cGCUUcAUUCGCAAGGCGUcuCGCu-- -3' miRNA: 3'- -CGGAcUGAGCGUUCCGCGuuGCGcgu -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 53768 | 0.66 | 0.593656 |
Target: 5'- uGCCgaaacGGUUUGCGAGcauugccgaGCGCAACGCGUAc -3' miRNA: 3'- -CGGa----CUGAGCGUUC---------CGCGUUGCGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 9511 | 0.67 | 0.582817 |
Target: 5'- gGUCgUGGCacaUCGCGAGGCcgGCAGC-CGCGa -3' miRNA: 3'- -CGG-ACUG---AGCGUUCCG--CGUUGcGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 9727 | 0.67 | 0.572019 |
Target: 5'- aGCCUgccagaggcGAUUCuGCGGGuuCGCAAUGCGCAu -3' miRNA: 3'- -CGGA---------CUGAG-CGUUCc-GCGUUGCGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 21195 | 0.67 | 0.572019 |
Target: 5'- cGCCcaUGG-UCGCAGGuauGCGCGGCGCGa- -3' miRNA: 3'- -CGG--ACUgAGCGUUC---CGCGUUGCGCgu -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 45788 | 0.67 | 0.561269 |
Target: 5'- aGCC-GGCgcggCGCGcGGC-CGACGCGUAc -3' miRNA: 3'- -CGGaCUGa---GCGUuCCGcGUUGCGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 20351 | 0.67 | 0.561269 |
Target: 5'- gGCU--ACUCGCAAaGCgGCGACGCGUc -3' miRNA: 3'- -CGGacUGAGCGUUcCG-CGUUGCGCGu -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 51422 | 0.67 | 0.550575 |
Target: 5'- cCCg----CGCAAGGCGUucCGCGCAc -3' miRNA: 3'- cGGacugaGCGUUCCGCGuuGCGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 26091 | 0.67 | 0.547379 |
Target: 5'- cGUC-GGCgUGCAGGGCGCGAUuaaccuugcggcggGCGCAc -3' miRNA: 3'- -CGGaCUGaGCGUUCCGCGUUG--------------CGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 29756 | 0.67 | 0.539944 |
Target: 5'- uUCUGGucCUCGuCAGGGcCGgAACGCGCu -3' miRNA: 3'- cGGACU--GAGC-GUUCC-GCgUUGCGCGu -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 50937 | 0.68 | 0.508501 |
Target: 5'- uGCCgcgucagcGAC-CGCAA-GCGCggUGCGCAc -3' miRNA: 3'- -CGGa-------CUGaGCGUUcCGCGuuGCGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 26556 | 0.68 | 0.498189 |
Target: 5'- uCCUGACcCGCcuu-CGCGACGCGCu -3' miRNA: 3'- cGGACUGaGCGuuccGCGUUGCGCGu -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 18309 | 0.68 | 0.477855 |
Target: 5'- uGCC-GAcCUCGCGAuGCGCGugGCGa- -3' miRNA: 3'- -CGGaCU-GAGCGUUcCGCGUugCGCgu -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 35239 | 0.68 | 0.477855 |
Target: 5'- uGCCggcaguacCUCGCGGcGGCGCcgcGACGCGUAc -3' miRNA: 3'- -CGGacu-----GAGCGUU-CCGCG---UUGCGCGU- -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 22119 | 0.69 | 0.457939 |
Target: 5'- gGCCgcauggGACggcaGCcaaaAAGGCGUGACGCGCc -3' miRNA: 3'- -CGGa-----CUGag--CG----UUCCGCGUUGCGCGu -5' |
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22623 | 3' | -57 | NC_005091.1 | + | 52034 | 0.69 | 0.457939 |
Target: 5'- aGCgUGACuuccucaaUCGCGAGauacGCGCcGCGCGCu -3' miRNA: 3'- -CGgACUG--------AGCGUUC----CGCGuUGCGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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