Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22632 | 3' | -47.8 | NC_005091.1 | + | 23356 | 0.66 | 0.97659 |
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Target: 5'- uCAGCCGgg-CGGCAUGUcGCgcagugcgUCGGc- -3' miRNA: 3'- -GUUGGUaaaGCCGUACA-CGa-------AGCUaa -5' |
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| 22632 | 3' | -47.8 | NC_005091.1 | + | 55443 | 0.66 | 0.973651 |
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Target: 5'- -uGCCAUccagUCGGCAcGU-CUUCGAUUc -3' miRNA: 3'- guUGGUAa---AGCCGUaCAcGAAGCUAA- -5' |
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| 22632 | 3' | -47.8 | NC_005091.1 | + | 9081 | 0.7 | 0.870504 |
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Target: 5'- uCGACCGUgggUCGGCGUcGUGCUgaagCGc-- -3' miRNA: 3'- -GUUGGUAa--AGCCGUA-CACGAa---GCuaa -5' |
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| 22632 | 3' | -47.8 | NC_005091.1 | + | 44715 | 0.71 | 0.843858 |
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Target: 5'- uGACCucgUCGGCcgaGUGCUUCGGc- -3' miRNA: 3'- gUUGGuaaAGCCGua-CACGAAGCUaa -5' |
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| 22632 | 3' | -47.8 | NC_005091.1 | + | 53698 | 0.73 | 0.706244 |
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Target: 5'- aAACCGUUUCGGCA--UGCgugCGAUg -3' miRNA: 3'- gUUGGUAAAGCCGUacACGaa-GCUAa -5' |
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| 22632 | 3' | -47.8 | NC_005091.1 | + | 46671 | 1.05 | 0.009715 |
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Target: 5'- uCAACCAUUUCGGCAUGUGCUUCGAUUc -3' miRNA: 3'- -GUUGGUAAAGCCGUACACGAAGCUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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