Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22713 | 3' | -56.6 | NC_005091.1 | + | 32033 | 0.66 | 0.648981 |
Target: 5'- -cGUCgccuugGCG-AGCGACGCG-AGCaGCa -3' miRNA: 3'- uuCAGa-----CGCaUCGCUGUGCgUCGcCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 6180 | 0.66 | 0.648981 |
Target: 5'- gGGGcUUGCG-GGCGACGaGCcgaAGCGGCg -3' miRNA: 3'- -UUCaGACGCaUCGCUGUgCG---UCGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 13888 | 0.66 | 0.637987 |
Target: 5'- cGGUCaGCacgacgcucGUuGCGGCA-GCAGCGGCc -3' miRNA: 3'- uUCAGaCG---------CAuCGCUGUgCGUCGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 41052 | 0.66 | 0.637987 |
Target: 5'- gGAGgc-GCGcAGCG-CACGCAGCgcugGGCg -3' miRNA: 3'- -UUCagaCGCaUCGCuGUGCGUCG----CCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 50620 | 0.66 | 0.626988 |
Target: 5'- -cGcCUGUGUuGaCG-UACGCGGCGGCc -3' miRNA: 3'- uuCaGACGCAuC-GCuGUGCGUCGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 15275 | 0.66 | 0.605013 |
Target: 5'- -cGUUUGUGaagAGCGGgucaACGCAcGCGGCc -3' miRNA: 3'- uuCAGACGCa--UCGCUg---UGCGU-CGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 9892 | 0.66 | 0.605013 |
Target: 5'- cGAG-CgcagGaUGUGGUGaauucGCGCGCAGCGGCc -3' miRNA: 3'- -UUCaGa---C-GCAUCGC-----UGUGCGUCGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 27329 | 0.66 | 0.594056 |
Target: 5'- cGGaCUGCGgcGCGACGC-CAGCcucuugauGGCc -3' miRNA: 3'- uUCaGACGCauCGCUGUGcGUCG--------CCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 52223 | 0.67 | 0.583131 |
Target: 5'- cGGUagUGCGgGGCGGCuuUGaCAGCGGCu -3' miRNA: 3'- uUCAg-ACGCaUCGCUGu-GC-GUCGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 52287 | 0.67 | 0.583131 |
Target: 5'- uAGUgUGCGUuGCGGaucgauuCGaCAGCGGCc -3' miRNA: 3'- uUCAgACGCAuCGCUgu-----GC-GUCGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 55416 | 0.67 | 0.561412 |
Target: 5'- cGGUCUcGCccacuucgAGCGcgaGCACGCGgGCGGCa -3' miRNA: 3'- uUCAGA-CGca------UCGC---UGUGCGU-CGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 43230 | 0.67 | 0.560331 |
Target: 5'- -cGUC-GCG-AGUGGCAUGCgaaagaaAGCGGCc -3' miRNA: 3'- uuCAGaCGCaUCGCUGUGCG-------UCGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 21394 | 0.67 | 0.549559 |
Target: 5'- ---cCUGaCG-GGCGGCcucgcguACGCGGCGGCg -3' miRNA: 3'- uucaGAC-GCaUCGCUG-------UGCGUCGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 6872 | 0.68 | 0.529277 |
Target: 5'- -uGUCUcCGUcGC-ACGCGCAGCGGa -3' miRNA: 3'- uuCAGAcGCAuCGcUGUGCGUCGCCg -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 24112 | 0.68 | 0.518712 |
Target: 5'- cGGUCUGCGUGGCGAucuguuugaaccCAuccacugcCGaCAGCGuGCc -3' miRNA: 3'- uUCAGACGCAUCGCU------------GU--------GC-GUCGC-CG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 17445 | 0.68 | 0.518712 |
Target: 5'- uGGUCUGCGU-GCGGaACuGCA-CGGCg -3' miRNA: 3'- uUCAGACGCAuCGCUgUG-CGUcGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 26400 | 0.68 | 0.497842 |
Target: 5'- cGGUCUGCu--GCGGCAgcCGCAGCcuGCg -3' miRNA: 3'- uUCAGACGcauCGCUGU--GCGUCGc-CG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 51577 | 0.68 | 0.477357 |
Target: 5'- -uGUCgUGCGUcGCGugacggGCGCGCAgaagcGCGGCa -3' miRNA: 3'- uuCAG-ACGCAuCGC------UGUGCGU-----CGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 24152 | 0.69 | 0.467271 |
Target: 5'- ---cUUGCGcagaUGGCGAaagAUGCAGCGGCg -3' miRNA: 3'- uucaGACGC----AUCGCUg--UGCGUCGCCG- -5' |
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22713 | 3' | -56.6 | NC_005091.1 | + | 3451 | 0.69 | 0.447438 |
Target: 5'- ---aUUGCagAGCGACACG-AGCGGCa -3' miRNA: 3'- uucaGACGcaUCGCUGUGCgUCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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