Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22719 | 5' | -55.7 | NC_005091.1 | + | 24572 | 0.67 | 0.660939 |
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Target: 5'- -cAGCGGugucGAUCGGGGCGgc-GUCGu -3' miRNA: 3'- gcUCGUCua--CUAGCCCCGCauaCGGC- -5' |
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| 22719 | 5' | -55.7 | NC_005091.1 | + | 46619 | 0.68 | 0.595593 |
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Target: 5'- cCGAGCAGcguUGAgcggaaGGGGCGa--GCCGu -3' miRNA: 3'- -GCUCGUCu--ACUag----CCCCGCauaCGGC- -5' |
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| 22719 | 5' | -55.7 | NC_005091.1 | + | 11063 | 0.69 | 0.535525 |
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Target: 5'- aGAGUAGaAUGGUuuugcugcgaucagaUGGGGaGUGUGCCGu -3' miRNA: 3'- gCUCGUC-UACUA---------------GCCCCgCAUACGGC- -5' |
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| 22719 | 5' | -55.7 | NC_005091.1 | + | 56693 | 0.69 | 0.531307 |
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Target: 5'- cCGGGguGAUGuggcAUCGGaGGCc-GUGCCGg -3' miRNA: 3'- -GCUCguCUAC----UAGCC-CCGcaUACGGC- -5' |
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| 22719 | 5' | -55.7 | NC_005091.1 | + | 33309 | 0.7 | 0.469705 |
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Target: 5'- gCGGGCAGAUGGUCGccGCGcauUAcGCCGg -3' miRNA: 3'- -GCUCGUCUACUAGCccCGC---AUaCGGC- -5' |
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| 22719 | 5' | -55.7 | NC_005091.1 | + | 44142 | 1.1 | 0.000879 |
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Target: 5'- cCGAGCAGAUGAUCGGGGCGUAUGCCGg -3' miRNA: 3'- -GCUCGUCUACUAGCCCCGCAUACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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