Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22721 | 3' | -59.8 | NC_005091.1 | + | 11017 | 0.76 | 0.127182 |
Target: 5'- cGCCGGGGuCGCuUUGCCGGgcgGCuuCCa -3' miRNA: 3'- cCGGCCCUuGCG-AACGGCCa--CGugGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 30293 | 0.66 | 0.529448 |
Target: 5'- cGUCGGugaaaucGAACgGCUUGcCCGGUGCGgcgugaacgaucuuCCCg -3' miRNA: 3'- cCGGCC-------CUUG-CGAAC-GGCCACGU--------------GGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 31282 | 0.66 | 0.50321 |
Target: 5'- aGGCCGgacGGAACGuCUUcGUCG-UGUAUCCa -3' miRNA: 3'- -CCGGC---CCUUGC-GAA-CGGCcACGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 54994 | 0.67 | 0.445015 |
Target: 5'- cGGCCGGGcg-GCccGCCGGUau-CCCg -3' miRNA: 3'- -CCGGCCCuugCGaaCGGCCAcguGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 16107 | 0.67 | 0.426464 |
Target: 5'- cGGaUCGGGGACGag-GCCaGccgcaugagaUGCACCCg -3' miRNA: 3'- -CC-GGCCCUUGCgaaCGGcC----------ACGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 51345 | 0.68 | 0.417362 |
Target: 5'- --gCGcGGAACGcCUUGCgCGGguucgugauuUGCGCCCg -3' miRNA: 3'- ccgGC-CCUUGC-GAACG-GCC----------ACGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 32104 | 0.68 | 0.417362 |
Target: 5'- cGGCCGGGuccGCGCcgacuuCCGGguUGCugCUg -3' miRNA: 3'- -CCGGCCCu--UGCGaac---GGCC--ACGugGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 34655 | 0.69 | 0.357107 |
Target: 5'- cGCCGGGGaugACGUUgaUGUCGG-GCAUCg -3' miRNA: 3'- cCGGCCCU---UGCGA--ACGGCCaCGUGGg -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 53174 | 0.7 | 0.317957 |
Target: 5'- cGCgCGGGcGCGagcCUU-CCGGUGCAUCCg -3' miRNA: 3'- cCG-GCCCuUGC---GAAcGGCCACGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 15169 | 0.7 | 0.289026 |
Target: 5'- cGGaUCGGGGACGagaccgGCCGcGUGCguugACCCg -3' miRNA: 3'- -CC-GGCCCUUGCgaa---CGGC-CACG----UGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 4683 | 0.7 | 0.289026 |
Target: 5'- gGGCCGGGGcCGUc-GCUGGUaGCucCCCg -3' miRNA: 3'- -CCGGCCCUuGCGaaCGGCCA-CGu-GGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 17553 | 0.71 | 0.255821 |
Target: 5'- cGGCCGGGAGCGU--GUCGacGCAaCCCc -3' miRNA: 3'- -CCGGCCCUUGCGaaCGGCcaCGU-GGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 43995 | 0.72 | 0.216855 |
Target: 5'- aGCUGGGAgaaguagacGCGCUUcucgauaugcaggucGCCGGgaagcgacUGCGCCCa -3' miRNA: 3'- cCGGCCCU---------UGCGAA---------------CGGCC--------ACGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 34347 | 0.76 | 0.127182 |
Target: 5'- cGGCCGGccGCGCUUGUCGuacUGCACgCCg -3' miRNA: 3'- -CCGGCCcuUGCGAACGGCc--ACGUG-GG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 44434 | 1.14 | 0.000194 |
Target: 5'- aGGCCGGGAACGCUUGCCGGUGCACCCg -3' miRNA: 3'- -CCGGCCCUUGCGAACGGCCACGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 8596 | 0.66 | 0.53353 |
Target: 5'- gGGCCGcagcccGGAUGCUcgaaCGcGUGCGCCCa -3' miRNA: 3'- -CCGGCc-----CUUGCGAacg-GC-CACGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 35747 | 0.66 | 0.50321 |
Target: 5'- aGCCGGGGcauccgcgcACGCgaGUCGGc-UACCCg -3' miRNA: 3'- cCGGCCCU---------UGCGaaCGGCCacGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 15386 | 0.66 | 0.483419 |
Target: 5'- cGGCaucgcaGGGGaaguuugucaauGCGUcugUUGUCGGUgaaGCGCCCa -3' miRNA: 3'- -CCGg-----CCCU------------UGCG---AACGGCCA---CGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 6835 | 0.67 | 0.473664 |
Target: 5'- -aCCGGGAcuucCGCgaccgUGCCGG-GCugCUu -3' miRNA: 3'- ccGGCCCUu---GCGa----ACGGCCaCGugGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 15855 | 0.67 | 0.464009 |
Target: 5'- -aCCGGGGGCGUcgGCaUGcGUGUugCCg -3' miRNA: 3'- ccGGCCCUUGCGaaCG-GC-CACGugGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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