Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22721 | 3' | -59.8 | NC_005091.1 | + | 28342 | 0.7 | 0.310525 |
Target: 5'- aGCCGccAGCGCgucGCCGGuUGCACCg -3' miRNA: 3'- cCGGCccUUGCGaa-CGGCC-ACGUGGg -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 16106 | 0.7 | 0.30979 |
Target: 5'- cGGCCGGcGGcagcacgACGCUUGUCGGccugaccgaUGUAgCCg -3' miRNA: 3'- -CCGGCC-CU-------UGCGAACGGCC---------ACGUgGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 15169 | 0.7 | 0.289026 |
Target: 5'- cGGaUCGGGGACGagaccgGCCGcGUGCguugACCCg -3' miRNA: 3'- -CC-GGCCCUUGCgaa---CGGC-CACG----UGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 4683 | 0.7 | 0.289026 |
Target: 5'- gGGCCGGGGcCGUc-GCUGGUaGCucCCCg -3' miRNA: 3'- -CCGGCCCUuGCGaaCGGCCA-CGu-GGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 17553 | 0.71 | 0.255821 |
Target: 5'- cGGCCGGGAGCGU--GUCGacGCAaCCCc -3' miRNA: 3'- -CCGGCCCUUGCGaaCGGCcaCGU-GGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 1240 | 0.72 | 0.231563 |
Target: 5'- cGCCGGGAugGCUgcacGCCaGUuagcguaauGCGCCUu -3' miRNA: 3'- cCGGCCCUugCGAa---CGGcCA---------CGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 43995 | 0.72 | 0.216855 |
Target: 5'- aGCUGGGAgaaguagacGCGCUUcucgauaugcaggucGCCGGgaagcgacUGCGCCCa -3' miRNA: 3'- cCGGCCCU---------UGCGAA---------------CGGCC--------ACGUGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 11017 | 0.76 | 0.127182 |
Target: 5'- cGCCGGGGuCGCuUUGCCGGgcgGCuuCCa -3' miRNA: 3'- cCGGCCCUuGCG-AACGGCCa--CGugGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 34347 | 0.76 | 0.127182 |
Target: 5'- cGGCCGGccGCGCUUGUCGuacUGCACgCCg -3' miRNA: 3'- -CCGGCCcuUGCGAACGGCc--ACGUG-GG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 10031 | 0.78 | 0.082313 |
Target: 5'- aGCCGGGGcCGUaauacUGCCGGUGCucGCCCu -3' miRNA: 3'- cCGGCCCUuGCGa----ACGGCCACG--UGGG- -5' |
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22721 | 3' | -59.8 | NC_005091.1 | + | 44434 | 1.14 | 0.000194 |
Target: 5'- aGGCCGGGAACGCUUGCCGGUGCACCCg -3' miRNA: 3'- -CCGGCCCUUGCGAACGGCCACGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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