Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22723 | 5' | -49.5 | NC_005091.1 | + | 44214 | 0.66 | 0.960422 |
Target: 5'- ---gCGGCGUcuUCGccuGCGUUgucUUCUGCUg -3' miRNA: 3'- uuaaGCCGCA--AGCu--UGCAA---AGGACGA- -5' |
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22723 | 5' | -49.5 | NC_005091.1 | + | 26282 | 0.67 | 0.925274 |
Target: 5'- --cUUGGCGUUCGcauCGUUcaccgcagCCUGCg -3' miRNA: 3'- uuaAGCCGCAAGCuu-GCAAa-------GGACGa -5' |
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22723 | 5' | -49.5 | NC_005091.1 | + | 28236 | 0.67 | 0.936805 |
Target: 5'- ---cCGGCGUgacgaUGAugGUUUCCggUGCa -3' miRNA: 3'- uuaaGCCGCAa----GCUugCAAAGG--ACGa -5' |
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22723 | 5' | -49.5 | NC_005091.1 | + | 25876 | 0.68 | 0.905012 |
Target: 5'- ----aGGCGUUCGGcuugcgccugcACGUauugguaUUCCUGCg -3' miRNA: 3'- uuaagCCGCAAGCU-----------UGCA-------AAGGACGa -5' |
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22723 | 5' | -49.5 | NC_005091.1 | + | 13821 | 0.68 | 0.875499 |
Target: 5'- -cUUCGGC--UCGGGCGguucgCCUGCg -3' miRNA: 3'- uuAAGCCGcaAGCUUGCaaa--GGACGa -5' |
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22723 | 5' | -49.5 | NC_005091.1 | + | 51801 | 0.69 | 0.840898 |
Target: 5'- ---cCGGCGUcCGGGCGUgcggCUUGCg -3' miRNA: 3'- uuaaGCCGCAaGCUUGCAaa--GGACGa -5' |
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22723 | 5' | -49.5 | NC_005091.1 | + | 46691 | 1.05 | 0.006961 |
Target: 5'- uAAUUCGGCGUUCGAACGUUUCCUGCUc -3' miRNA: 3'- -UUAAGCCGCAAGCUUGCAAAGGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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