miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22723 5' -49.5 NC_005091.1 + 13821 0.68 0.875499
Target:  5'- -cUUCGGC--UCGGGCGguucgCCUGCg -3'
miRNA:   3'- uuAAGCCGcaAGCUUGCaaa--GGACGa -5'
22723 5' -49.5 NC_005091.1 + 25876 0.68 0.905012
Target:  5'- ----aGGCGUUCGGcuugcgccugcACGUauugguaUUCCUGCg -3'
miRNA:   3'- uuaagCCGCAAGCU-----------UGCA-------AAGGACGa -5'
22723 5' -49.5 NC_005091.1 + 26282 0.67 0.925274
Target:  5'- --cUUGGCGUUCGcauCGUUcaccgcagCCUGCg -3'
miRNA:   3'- uuaAGCCGCAAGCuu-GCAAa-------GGACGa -5'
22723 5' -49.5 NC_005091.1 + 28236 0.67 0.936805
Target:  5'- ---cCGGCGUgacgaUGAugGUUUCCggUGCa -3'
miRNA:   3'- uuaaGCCGCAa----GCUugCAAAGG--ACGa -5'
22723 5' -49.5 NC_005091.1 + 44214 0.66 0.960422
Target:  5'- ---gCGGCGUcuUCGccuGCGUUgucUUCUGCUg -3'
miRNA:   3'- uuaaGCCGCA--AGCu--UGCAA---AGGACGA- -5'
22723 5' -49.5 NC_005091.1 + 46691 1.05 0.006961
Target:  5'- uAAUUCGGCGUUCGAACGUUUCCUGCUc -3'
miRNA:   3'- -UUAAGCCGCAAGCUUGCAAAGGACGA- -5'
22723 5' -49.5 NC_005091.1 + 51801 0.69 0.840898
Target:  5'- ---cCGGCGUcCGGGCGUgcggCUUGCg -3'
miRNA:   3'- uuaaGCCGCAaGCUUGCAaa--GGACGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.