Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22728 | 5' | -50.1 | NC_005091.1 | + | 30291 | 0.66 | 0.960984 |
Target: 5'- gUCGUCGGUGAAAucgaacggcUUGCCCgguGCGGcGUGa -3' miRNA: 3'- -GGUAGUUACUUUu--------AGCGGG---UGCC-CGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 11657 | 0.66 | 0.954429 |
Target: 5'- gCCGUCA--GGAAGUCGCCgGCcuucagcggcucgauGGcGCGg -3' miRNA: 3'- -GGUAGUuaCUUUUAGCGGgUG---------------CC-CGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 46913 | 0.66 | 0.95268 |
Target: 5'- uCCGUUcauGAUGAAcgucUCGUCCuguuCGGGCa -3' miRNA: 3'- -GGUAG---UUACUUuu--AGCGGGu---GCCCGc -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 26448 | 0.66 | 0.948113 |
Target: 5'- cCCGUCAG-GAc---CGCgCGCGGGCc -3' miRNA: 3'- -GGUAGUUaCUuuuaGCGgGUGCCCGc -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 30407 | 0.66 | 0.943264 |
Target: 5'- aCCcgCGAcggGAAGAUCGUUCAcgccgcacCGGGCa -3' miRNA: 3'- -GGuaGUUa--CUUUUAGCGGGU--------GCCCGc -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 31580 | 0.67 | 0.938128 |
Target: 5'- aCgAUCGAUGAAcaccgGcgUGCCCGCGaaGGCu -3' miRNA: 3'- -GgUAGUUACUU-----UuaGCGGGUGC--CCGc -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 22315 | 0.67 | 0.937598 |
Target: 5'- aCCGUCAG-----GUCGCUCGugucgucCGGGCGa -3' miRNA: 3'- -GGUAGUUacuuuUAGCGGGU-------GCCCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 30530 | 0.67 | 0.920984 |
Target: 5'- gCGUC-AUGAGcAUCGCCacggACGGGUc -3' miRNA: 3'- gGUAGuUACUUuUAGCGGg---UGCCCGc -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 34868 | 0.67 | 0.914689 |
Target: 5'- cUCAUCAAcccgcUGAAgcgGAUCGCCgACGcGcGCGg -3' miRNA: 3'- -GGUAGUU-----ACUU---UUAGCGGgUGC-C-CGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 51273 | 0.68 | 0.908106 |
Target: 5'- aUCGUCGccGucg--CGCCCGUGGGCGa -3' miRNA: 3'- -GGUAGUuaCuuuuaGCGGGUGCCCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 5198 | 0.68 | 0.891885 |
Target: 5'- gCCGUCAcgGAAGAaguacgggcCGCCCAUgucacgccccgcagGGGCa -3' miRNA: 3'- -GGUAGUuaCUUUUa--------GCGGGUG--------------CCCGc -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 38892 | 0.68 | 0.886654 |
Target: 5'- gCGUCGcgGUGAGAAgaugcaggCGCUugauUACGGGCGa -3' miRNA: 3'- gGUAGU--UACUUUUa-------GCGG----GUGCCCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 37135 | 0.68 | 0.886654 |
Target: 5'- aCgGUCucguUGAAccAAUCGCCCACGuacgcgcccGGCGu -3' miRNA: 3'- -GgUAGuu--ACUU--UUAGCGGGUGC---------CCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 57358 | 0.68 | 0.886654 |
Target: 5'- aCgGUCGauGUGAAGAU-GCUCgaaGCGGGCGg -3' miRNA: 3'- -GgUAGU--UACUUUUAgCGGG---UGCCCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 36610 | 0.7 | 0.81798 |
Target: 5'- gCCGUCGAuacUGGAAGU-GCCCGCacugcGGCGa -3' miRNA: 3'- -GGUAGUU---ACUUUUAgCGGGUGc----CCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 37247 | 0.7 | 0.80837 |
Target: 5'- -gGUCGAUGAccAUCGCCC-CGaGCGa -3' miRNA: 3'- ggUAGUUACUuuUAGCGGGuGCcCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 21619 | 0.71 | 0.747041 |
Target: 5'- cCCcgCAAgc-GGAUCGaCCgGCGGGCGg -3' miRNA: 3'- -GGuaGUUacuUUUAGC-GGgUGCCCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 47064 | 0.73 | 0.647611 |
Target: 5'- gCCAgUCGAUGAAGAUgccgCGCUCGCGGucaGCGg -3' miRNA: 3'- -GGU-AGUUACUUUUA----GCGGGUGCC---CGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 6598 | 0.74 | 0.602522 |
Target: 5'- -aGUCGAUGA---UCGCUCGCGcGGCGu -3' miRNA: 3'- ggUAGUUACUuuuAGCGGGUGC-CCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 15352 | 0.74 | 0.591292 |
Target: 5'- -uGUCGGUGAAg--CGCCCACGGaCGg -3' miRNA: 3'- ggUAGUUACUUuuaGCGGGUGCCcGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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