Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22730 | 3' | -53.2 | NC_005091.1 | + | 36810 | 0.66 | 0.809481 |
Target: 5'- -----gACGACGUUgaUGGCgacgGCGAGCCg -3' miRNA: 3'- cagagaUGUUGCGA--GCCGa---UGCUCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 23472 | 0.66 | 0.828195 |
Target: 5'- gGUCUgUucgGCGGCGCugcgUCGGCUGCcccGGCUg -3' miRNA: 3'- -CAGAgA---UGUUGCG----AGCCGAUGc--UCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 26489 | 0.66 | 0.83724 |
Target: 5'- -----gGCAACGCa-GGCUGCGGcuGCCg -3' miRNA: 3'- cagagaUGUUGCGagCCGAUGCU--CGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 36081 | 0.66 | 0.846065 |
Target: 5'- --gUCUGCAACGUguUCGGCauUGcGCCg -3' miRNA: 3'- cagAGAUGUUGCG--AGCCGauGCuCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 26464 | 0.66 | 0.846065 |
Target: 5'- -gCUCUGCc-UGCUCGGCgacuUGCGcuucaaGGCCa -3' miRNA: 3'- caGAGAUGuuGCGAGCCG----AUGC------UCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 2466 | 0.66 | 0.846065 |
Target: 5'- -----aGCAagGCGUUCGGCUACGGGa- -3' miRNA: 3'- cagagaUGU--UGCGAGCCGAUGCUCgg -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 33754 | 0.66 | 0.818938 |
Target: 5'- cGUCaUC-GCGAUGC-CGGCUugGAuGCUc -3' miRNA: 3'- -CAG-AGaUGUUGCGaGCCGAugCU-CGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 52003 | 0.66 | 0.809481 |
Target: 5'- gGUCggcgCUACGGCGUcgcauuUCGGg-GCGAGCg -3' miRNA: 3'- -CAGa---GAUGUUGCG------AGCCgaUGCUCGg -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 52327 | 0.67 | 0.759567 |
Target: 5'- cGUCgcaUGCGACGUUgaGGCgacggGCGAGCa -3' miRNA: 3'- -CAGag-AUGUUGCGAg-CCGa----UGCUCGg -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 49822 | 0.67 | 0.759567 |
Target: 5'- uGUCUCgUACGacucACGCaCGGCcgACGuGCCc -3' miRNA: 3'- -CAGAG-AUGU----UGCGaGCCGa-UGCuCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 26883 | 0.67 | 0.759567 |
Target: 5'- uGUCaggcgCUGCugauGCGCUCgaucaGGCgaagacGCGAGCCg -3' miRNA: 3'- -CAGa----GAUGu---UGCGAG-----CCGa-----UGCUCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 56917 | 0.67 | 0.803715 |
Target: 5'- uUCUCUGCAcgaucggcaauugccGuCGCU-GGCUGCGcggugcAGCCa -3' miRNA: 3'- cAGAGAUGU---------------U-GCGAgCCGAUGC------UCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 35463 | 0.68 | 0.738586 |
Target: 5'- uGUCcgUACGcCGCUCGGagACGAGCUg -3' miRNA: 3'- -CAGagAUGUuGCGAGCCgaUGCUCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 31190 | 0.68 | 0.728997 |
Target: 5'- cUC-CUGCAACGacaugccgccgaugaUCGGCUGCucGCCg -3' miRNA: 3'- cAGaGAUGUUGCg--------------AGCCGAUGcuCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 50788 | 0.68 | 0.717168 |
Target: 5'- -gCUUgGCAACGCUCGGCgcGCGuuGCa -3' miRNA: 3'- caGAGaUGUUGCGAGCCGa-UGCu-CGg -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 18978 | 0.68 | 0.749138 |
Target: 5'- -----cGCGAUGCUCGGCggaauCGuGCCg -3' miRNA: 3'- cagagaUGUUGCGAGCCGau---GCuCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 19629 | 0.68 | 0.727926 |
Target: 5'- uGUCg--GCGGCGCUCGGCccgaaaGCGcAGCa -3' miRNA: 3'- -CAGagaUGUUGCGAGCCGa-----UGC-UCGg -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 25603 | 0.68 | 0.706324 |
Target: 5'- uUCUCggcuucgcGCAACaGCUCGGCgAUGAGUa -3' miRNA: 3'- cAGAGa-------UGUUG-CGAGCCGaUGCUCGg -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 11551 | 0.68 | 0.695405 |
Target: 5'- aUC-CU-CGGCGCauUCGGCUAC-AGCCg -3' miRNA: 3'- cAGaGAuGUUGCG--AGCCGAUGcUCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 46217 | 0.68 | 0.695405 |
Target: 5'- cGUCUCguaaucauCGGCGUcCGGC-ACGAGCa -3' miRNA: 3'- -CAGAGau------GUUGCGaGCCGaUGCUCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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