Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22834 | 3' | -45.4 | NC_005135.1 | + | 172227 | 0.8 | 0.804005 |
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Target: 5'- -uUACAAAAA-GGcCCUCCGAAGAGGg -3' miRNA: 3'- uuAUGUUUUUaUC-GGAGGCUUCUCCg -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 34858 | 0.85 | 0.597575 |
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Target: 5'- cGUACAAucauaauaaacuccAAAUgugGGCCUCCGGAGAGGCc -3' miRNA: 3'- uUAUGUU--------------UUUA---UCGGAGGCUUCUCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 73477 | 0.86 | 0.535318 |
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Target: 5'- -uUACAAAAAUGcCCUCCGAAGAGGg -3' miRNA: 3'- uuAUGUUUUUAUcGGAGGCUUCUCCg -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 115842 | 0.87 | 0.462305 |
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Target: 5'- cAUACAAAAAUGcCCUCCGAAGAGGg -3' miRNA: 3'- uUAUGUUUUUAUcGGAGGCUUCUCCg -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 166604 | 0.88 | 0.423 |
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Target: 5'- --aACAAA---GGCCUCCGAAGAGGCc -3' miRNA: 3'- uuaUGUUUuuaUCGGAGGCUUCUCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 4214 | 0.88 | 0.423 |
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Target: 5'- --aACAAA---GGCCUCCGAAGAGGCc -3' miRNA: 3'- uuaUGUUUuuaUCGGAGGCUUCUCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 64059 | 1.11 | 0.022866 |
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Target: 5'- gAAUACAAAAAUAGCCUCCGAAGAGGCu -3' miRNA: 3'- -UUAUGUUUUUAUCGGAGGCUUCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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