Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22834 | 3' | -45.4 | NC_005135.1 | + | 42226 | 0.68 | 0.99981 |
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Target: 5'- ---cCGAA---AGcCCUCCGAAGAGGg -3' miRNA: 3'- uuauGUUUuuaUC-GGAGGCUUCUCCg -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 30227 | 0.68 | 0.999854 |
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Target: 5'- uAUACGAcAAaGGCCUCacuuGGAGGCc -3' miRNA: 3'- uUAUGUUuUUaUCGGAGgcu-UCUCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 166803 | 0.67 | 0.999937 |
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Target: 5'- ---cCAAAA--GGCCUCUucGGAGGCc -3' miRNA: 3'- uuauGUUUUuaUCGGAGGcuUCUCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 43248 | 0.67 | 0.999965 |
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Target: 5'- cAAUGCAuucgcgacuuAGGGUGGCgaCCGucGAGGUg -3' miRNA: 3'- -UUAUGU----------UUUUAUCGgaGGCuuCUCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 65816 | 0.66 | 0.999991 |
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Target: 5'- uAAUGCGcAGGAUGGCC----AAGAGGCa -3' miRNA: 3'- -UUAUGU-UUUUAUCGGaggcUUCUCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 154704 | 0.66 | 0.999991 |
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Target: 5'- ---cCAAAGG-AGCC-CCGAAG-GGCu -3' miRNA: 3'- uuauGUUUUUaUCGGaGGCUUCuCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 82608 | 0.66 | 0.999994 |
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Target: 5'- --cGCAAAAAUAGUaucUUCCGuugGGAGaGCu -3' miRNA: 3'- uuaUGUUUUUAUCG---GAGGCu--UCUC-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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