Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22834 | 3' | -45.4 | NC_005135.1 | + | 30227 | 0.68 | 0.999854 |
|
Target: 5'- uAUACGAcAAaGGCCUCacuuGGAGGCc -3' miRNA: 3'- uUAUGUUuUUaUCGGAGgcu-UCUCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 28817 | 0.73 | 0.989397 |
|
Target: 5'- uAUACAAAAAUAGgCUCCGuAGAGc- -3' miRNA: 3'- uUAUGUUUUUAUCgGAGGCuUCUCcg -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 25909 | 0.68 | 0.999755 |
|
Target: 5'- ---uCGAAuaucuGCCUCCGAAaAGGCa -3' miRNA: 3'- uuauGUUUuuau-CGGAGGCUUcUCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 16254 | 0.78 | 0.90375 |
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Target: 5'- --aACAAAAAgacCCUCCGAAGAGGg -3' miRNA: 3'- uuaUGUUUUUaucGGAGGCUUCUCCg -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 11304 | 0.69 | 0.999601 |
|
Target: 5'- --gACAAAAAUAGUUUuuGggGAaGCa -3' miRNA: 3'- uuaUGUUUUUAUCGGAggCuuCUcCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 4416 | 0.73 | 0.987863 |
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Target: 5'- --gACGAAAA-GGCCUCUucGGAGGCc -3' miRNA: 3'- uuaUGUUUUUaUCGGAGGcuUCUCCG- -5' |
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| 22834 | 3' | -45.4 | NC_005135.1 | + | 4214 | 0.88 | 0.423 |
|
Target: 5'- --aACAAA---GGCCUCCGAAGAGGCc -3' miRNA: 3'- uuaUGUUUuuaUCGGAGGCUUCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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