Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22855 | 5' | -63.5 | NC_005137.2 | + | 21569 | 0.66 | 0.596947 |
Target: 5'- gCGUUGGGUGaccgauGGGcCGCGcauaGGCCGCg -3' miRNA: 3'- -GCAGCCCGCg-----CCC-GUGCag--CCGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 100251 | 0.66 | 0.596947 |
Target: 5'- --cCGGGCGCcgcaacgcacGGGCGCGcgucgCGGCauucaCGCCc -3' miRNA: 3'- gcaGCCCGCG----------CCCGUGCa----GCCG-----GUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 100044 | 0.66 | 0.596947 |
Target: 5'- cCGaCGcGGCGCGcGGCGCGUucagcaugcuuUGuGCCGCg -3' miRNA: 3'- -GCaGC-CCGCGC-CCGUGCA-----------GC-CGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 61365 | 0.66 | 0.596947 |
Target: 5'- gCGUCaaauGGGCgGCcGGCgaguugaugGCGUUGGCgGCCg -3' miRNA: 3'- -GCAG----CCCG-CGcCCG---------UGCAGCCGgUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 56498 | 0.66 | 0.596947 |
Target: 5'- gCGUCGugucGCGUGuacGGCguuACGUUGGCCGCa -3' miRNA: 3'- -GCAGCc---CGCGC---CCG---UGCAGCCGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 128651 | 0.66 | 0.587318 |
Target: 5'- cCGUCGGGUaCGuacacuuGCACGUuuaCGGCCAUg -3' miRNA: 3'- -GCAGCCCGcGCc------CGUGCA---GCCGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 22840 | 0.66 | 0.587318 |
Target: 5'- aCG-CGaGG-GCGGGCGCG-CGcauaccgacGCCGCCg -3' miRNA: 3'- -GCaGC-CCgCGCCCGUGCaGC---------CGGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 119851 | 0.66 | 0.587318 |
Target: 5'- -aUCGGGCGUGguGGCACGcgCaGCCAa- -3' miRNA: 3'- gcAGCCCGCGC--CCGUGCa-GcCGGUgg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 24396 | 0.66 | 0.558625 |
Target: 5'- uGUUGGaCGCGG---CGUCaGGCCGCCg -3' miRNA: 3'- gCAGCCcGCGCCcguGCAG-CCGGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 98147 | 0.66 | 0.558625 |
Target: 5'- -uUCGGcCGCGccuuuuauagccGGCGCG-CGGCCACa -3' miRNA: 3'- gcAGCCcGCGC------------CCGUGCaGCCGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 45111 | 0.66 | 0.549145 |
Target: 5'- uCG-CGGGCGCGcGaCGCGUCGcacaacuuaaCCACCa -3' miRNA: 3'- -GCaGCCCGCGCcC-GUGCAGCc---------GGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 34598 | 0.67 | 0.539716 |
Target: 5'- gCGUCGccGCGCGGGUAgcgGUCGGCgCGgCu -3' miRNA: 3'- -GCAGCc-CGCGCCCGUg--CAGCCG-GUgG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 38475 | 0.67 | 0.530342 |
Target: 5'- aCGUUuaaaaGCGUaaacuauuucGGGCACGUUGGcCCGCCg -3' miRNA: 3'- -GCAGcc---CGCG----------CCCGUGCAGCC-GGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 69607 | 0.67 | 0.530342 |
Target: 5'- --aCGcGGCGUuucuUACGUCGGCCGCCa -3' miRNA: 3'- gcaGC-CCGCGccc-GUGCAGCCGGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 71096 | 0.67 | 0.521028 |
Target: 5'- uGUCGGGCG-Ga--GCGUUGGCgGCCc -3' miRNA: 3'- gCAGCCCGCgCccgUGCAGCCGgUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 75964 | 0.67 | 0.502598 |
Target: 5'- uGUCGuGaCGCGGcGCGCGUUGGUgCACg -3' miRNA: 3'- gCAGCcC-GCGCC-CGUGCAGCCG-GUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 12879 | 0.67 | 0.493489 |
Target: 5'- --aCGGGCGCGGG-GCGaaacaGGUgGCCg -3' miRNA: 3'- gcaGCCCGCGCCCgUGCag---CCGgUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 34978 | 0.67 | 0.492583 |
Target: 5'- uGUCGcguucccguuuguGGCGaCGGGCAa--CGGCCugCa -3' miRNA: 3'- gCAGC-------------CCGC-GCCCGUgcaGCCGGugG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 26557 | 0.68 | 0.484458 |
Target: 5'- aGUCGuGGCGCuGcGGCGUGUCGaCUGCCa -3' miRNA: 3'- gCAGC-CCGCG-C-CCGUGCAGCcGGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 22438 | 0.68 | 0.466637 |
Target: 5'- aCGUC-GGCGauuuGGGCAacgugcgcucCGUCGGCugCACCg -3' miRNA: 3'- -GCAGcCCGCg---CCCGU----------GCAGCCG--GUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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