Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22855 | 5' | -63.5 | NC_005137.2 | + | 128651 | 0.66 | 0.587318 |
Target: 5'- cCGUCGGGUaCGuacacuuGCACGUuuaCGGCCAUg -3' miRNA: 3'- -GCAGCCCGcGCc------CGUGCA---GCCGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 128567 | 1.11 | 0.000475 |
Target: 5'- uCGUCGGGCGCGGGCACGUCGGCCACCg -3' miRNA: 3'- -GCAGCCCGCGCCCGUGCAGCCGGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 119851 | 0.66 | 0.587318 |
Target: 5'- -aUCGGGCGUGguGGCACGcgCaGCCAa- -3' miRNA: 3'- gcAGCCCGCGC--CCGUGCa-GcCGGUgg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 112686 | 0.8 | 0.08356 |
Target: 5'- gGUCGGGacgcacguuaaaCGCGGGCACGUCGuCCACg -3' miRNA: 3'- gCAGCCC------------GCGCCCGUGCAGCcGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 109409 | 0.72 | 0.271671 |
Target: 5'- cCGUCGGGCucgucaaaaccGCGGccuauGCGCGgcccaUCGGUCACCc -3' miRNA: 3'- -GCAGCCCG-----------CGCC-----CGUGC-----AGCCGGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 108146 | 0.73 | 0.231757 |
Target: 5'- uCGUCGGGCgGCGGcgGCGUCGGuaugcgcgcgcCCGCCc -3' miRNA: 3'- -GCAGCCCG-CGCCcgUGCAGCC-----------GGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 102949 | 0.7 | 0.375317 |
Target: 5'- cCGUCuGGCGCaucccaGcGCGCGUCGcGCCACg -3' miRNA: 3'- -GCAGcCCGCGc-----C-CGUGCAGC-CGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 100251 | 0.66 | 0.596947 |
Target: 5'- --cCGGGCGCcgcaacgcacGGGCGCGcgucgCGGCauucaCGCCc -3' miRNA: 3'- gcaGCCCGCG----------CCCGUGCa----GCCG-----GUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 100044 | 0.66 | 0.596947 |
Target: 5'- cCGaCGcGGCGCGcGGCGCGUucagcaugcuuUGuGCCGCg -3' miRNA: 3'- -GCaGC-CCGCGC-CCGUGCA-----------GC-CGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 99372 | 0.71 | 0.330859 |
Target: 5'- gCGUCcGcGCGCGuGCAcugacCGUUGGCCGCCa -3' miRNA: 3'- -GCAGcC-CGCGCcCGU-----GCAGCCGGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 98147 | 0.66 | 0.558625 |
Target: 5'- -uUCGGcCGCGccuuuuauagccGGCGCG-CGGCCACa -3' miRNA: 3'- gcAGCCcGCGC------------CCGUGCaGCCGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 98030 | 0.69 | 0.426155 |
Target: 5'- aCGUCGGGCgcugcgaGCGGGUccagcagcgccuuucGCGcaaaGGCCGCa -3' miRNA: 3'- -GCAGCCCG-------CGCCCG---------------UGCag--CCGGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 97987 | 0.68 | 0.457854 |
Target: 5'- --aCGGGCGCGcGGCGUGggugCGcCCGCCa -3' miRNA: 3'- gcaGCCCGCGC-CCGUGCa---GCcGGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 91190 | 0.7 | 0.337274 |
Target: 5'- --cCGuGGCGCGGcguuugguugcacGCGUGUUGGCCGCCa -3' miRNA: 3'- gcaGC-CCGCGCC-------------CGUGCAGCCGGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 89394 | 0.74 | 0.206364 |
Target: 5'- gGUCGGGCGUauGGGCuuGUaCGG-CACCa -3' miRNA: 3'- gCAGCCCGCG--CCCGugCA-GCCgGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 87189 | 0.71 | 0.310128 |
Target: 5'- uCGaCGGGCGCGGGCGgcUGcUCGuGCCGUCg -3' miRNA: 3'- -GCaGCCCGCGCCCGU--GC-AGC-CGGUGG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 84568 | 0.76 | 0.147751 |
Target: 5'- aCGUCGGcGCGUucGGGCGgGUCGGgCACg -3' miRNA: 3'- -GCAGCC-CGCG--CCCGUgCAGCCgGUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 80262 | 0.71 | 0.323837 |
Target: 5'- uGUCuGGCaacGCGGGCAUGUCGcGUUugCa -3' miRNA: 3'- gCAGcCCG---CGCCCGUGCAGC-CGGugG- -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 75964 | 0.67 | 0.502598 |
Target: 5'- uGUCGuGaCGCGGcGCGCGUUGGUgCACg -3' miRNA: 3'- gCAGCcC-GCGCC-CGUGCAGCCG-GUGg -5' |
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22855 | 5' | -63.5 | NC_005137.2 | + | 75014 | 0.69 | 0.399012 |
Target: 5'- -aUCGaGCGgGGuGC-CGUCGGCCACg -3' miRNA: 3'- gcAGCcCGCgCC-CGuGCAGCCGGUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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