Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22856 | 3' | -49 | NC_005137.2 | + | 31249 | 0.66 | 0.998338 |
Target: 5'- uCGCGCGgc--CAAAcaauuAGu--GCUCGCCa -3' miRNA: 3'- -GCGCGCaguaGUUU-----UCuuuCGAGCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 61416 | 0.66 | 0.998338 |
Target: 5'- aCGcCGCGUUcUCAAAcaugucGGGAcGUUUGCCg -3' miRNA: 3'- -GC-GCGCAGuAGUUU------UCUUuCGAGCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 95882 | 0.66 | 0.998338 |
Target: 5'- gCGCGUGUCAaaccuUCGGuguuGAccGCguuugCGCCa -3' miRNA: 3'- -GCGCGCAGU-----AGUUuu--CUuuCGa----GCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 81554 | 0.66 | 0.998338 |
Target: 5'- aCGCuCGUUGUUAAuAGccAAGCUCGCg -3' miRNA: 3'- -GCGcGCAGUAGUUuUCu-UUCGAGCGg -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 20192 | 0.66 | 0.998 |
Target: 5'- uGCGCGUCGgcgggcggcgUCGAuugcGAAAGCgcguaCGCUu -3' miRNA: 3'- gCGCGCAGU----------AGUUuu--CUUUCGa----GCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 42883 | 0.66 | 0.99777 |
Target: 5'- uCGCGUGUCcgUu---GAGGGCguguuuucacacgcgUCGCCg -3' miRNA: 3'- -GCGCGCAGuaGuuuuCUUUCG---------------AGCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 108627 | 0.66 | 0.997605 |
Target: 5'- gGUGCGUaggGUUAAAGu---GCUCGCCu -3' miRNA: 3'- gCGCGCAg--UAGUUUUcuuuCGAGCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 42382 | 0.66 | 0.997605 |
Target: 5'- aGCGCGUCAaguguugCAAGuu-GAGCUucggcggugcccCGCCg -3' miRNA: 3'- gCGCGCAGUa------GUUUucuUUCGA------------GCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 104690 | 0.66 | 0.997605 |
Target: 5'- aCGCGCGU-GUUAGAGuccAGCgCGCCg -3' miRNA: 3'- -GCGCGCAgUAGUUUUcuuUCGaGCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 107533 | 0.66 | 0.997562 |
Target: 5'- gCGUGCGUCAUUAaaAAAGAAgaaaacguugagcAGUcCGCa -3' miRNA: 3'- -GCGCGCAGUAGU--UUUCUU-------------UCGaGCGg -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 22827 | 0.66 | 0.997148 |
Target: 5'- uGCGCGUUGcCAAAcgcGAGGGCgggCGCg -3' miRNA: 3'- gCGCGCAGUaGUUUu--CUUUCGa--GCGg -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 9925 | 0.66 | 0.997148 |
Target: 5'- uGCGCGUCAUCAAAcu---GC-CGUUu -3' miRNA: 3'- gCGCGCAGUAGUUUucuuuCGaGCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 39828 | 0.66 | 0.997148 |
Target: 5'- aCGcCGCGcCA-CGGAAGAAAGCgaguuuagCGCa -3' miRNA: 3'- -GC-GCGCaGUaGUUUUCUUUCGa-------GCGg -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 91391 | 0.66 | 0.997148 |
Target: 5'- gGCG-GaCGUUgcGAGAAAGUUUGCCg -3' miRNA: 3'- gCGCgCaGUAGuuUUCUUUCGAGCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 23568 | 0.66 | 0.996945 |
Target: 5'- aCGUGCGUguUCuucccacGGCUCGUCg -3' miRNA: 3'- -GCGCGCAguAGuuuucuuUCGAGCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 5129 | 0.66 | 0.99662 |
Target: 5'- aCGCGCGcCGaCAAuuaaacuuGGAAGaaaUCGCCa -3' miRNA: 3'- -GCGCGCaGUaGUUuu------CUUUCg--AGCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 31404 | 0.66 | 0.99662 |
Target: 5'- uGCGCGUCAcCAAccguGgcAGCUgcacgucgaCGCCg -3' miRNA: 3'- gCGCGCAGUaGUUuu--CuuUCGA---------GCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 52899 | 0.66 | 0.99662 |
Target: 5'- gGUGCGUCG-CAAGGGAAA-CU-GCCc -3' miRNA: 3'- gCGCGCAGUaGUUUUCUUUcGAgCGG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 81806 | 0.67 | 0.996014 |
Target: 5'- uCGCGCGUCGcguaUCGAugugcccaccGAGAGCUUGaCg -3' miRNA: 3'- -GCGCGCAGU----AGUUuu--------CUUUCGAGCgG- -5' |
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22856 | 3' | -49 | NC_005137.2 | + | 115925 | 0.67 | 0.996014 |
Target: 5'- aUGCGCGUUguggaauguGUCAAGAGugugGAAGCgguuagCGCg -3' miRNA: 3'- -GCGCGCAG---------UAGUUUUC----UUUCGa-----GCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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