Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22856 | 5' | -56 | NC_005137.2 | + | 85250 | 0.73 | 0.541503 |
Target: 5'- aUCGGCGuCGCUGUCgUCAGcggcguaacccuUGgCGCGGCu -3' miRNA: 3'- -AGCUGC-GCGGCAG-AGUU------------ACaGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 48002 | 0.66 | 0.912181 |
Target: 5'- aUGACGCGCUcacaacUCgUCAAUGguaUCGCGGa -3' miRNA: 3'- aGCUGCGCGGc-----AG-AGUUAC---AGCGCCg -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 31198 | 0.66 | 0.912181 |
Target: 5'- cCGGCccGCGUCGccCUUguaccaGcgGUCGCGGCa -3' miRNA: 3'- aGCUG--CGCGGCa-GAG------UuaCAGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 129980 | 0.66 | 0.906136 |
Target: 5'- cCGACuccagaagguaCGuuGUCUCGcgG-CGCGGCu -3' miRNA: 3'- aGCUGc----------GCggCAGAGUuaCaGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 68393 | 0.66 | 0.899858 |
Target: 5'- -gGACGCGUCGgccgagcacaUCGAcuUGUaCGUGGCg -3' miRNA: 3'- agCUGCGCGGCag--------AGUU--ACA-GCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 56355 | 0.66 | 0.899858 |
Target: 5'- aCGACGCGguaacCCGUCgaccgCGAUGU--UGGCg -3' miRNA: 3'- aGCUGCGC-----GGCAGa----GUUACAgcGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 71070 | 0.66 | 0.893349 |
Target: 5'- cUCGAUGCgaaauuGCUGcaaaUCAAUGUCG-GGCg -3' miRNA: 3'- -AGCUGCG------CGGCag--AGUUACAGCgCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 72805 | 0.66 | 0.877522 |
Target: 5'- gCGGCGCgcgcuGCCGUCUUGAUGUUuagcauguaaaugaGCGcGUa -3' miRNA: 3'- aGCUGCG-----CGGCAGAGUUACAG--------------CGC-CG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 114807 | 0.67 | 0.841672 |
Target: 5'- gCGcACGuCGUCGUCgucaGAUGUCauagGCGGCu -3' miRNA: 3'- aGC-UGC-GCGGCAGag--UUACAG----CGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 18573 | 0.68 | 0.807848 |
Target: 5'- gCGAcCGCGCCGUUUCAA--UUGCGa- -3' miRNA: 3'- aGCU-GCGCGGCAGAGUUacAGCGCcg -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 44256 | 0.68 | 0.789043 |
Target: 5'- gUUGACGCGCaCGUaacguucCUCAAgagcGUUGCGcGCu -3' miRNA: 3'- -AGCUGCGCG-GCA-------GAGUUa---CAGCGC-CG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 31464 | 0.69 | 0.752539 |
Target: 5'- aCaACGCGuuGUCUU-GUG-CGCGGCa -3' miRNA: 3'- aGcUGCGCggCAGAGuUACaGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 97431 | 0.69 | 0.742899 |
Target: 5'- -gGGCGCGgCGcaCUCAaaaacAUGUCGgGGCa -3' miRNA: 3'- agCUGCGCgGCa-GAGU-----UACAGCgCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 100049 | 0.7 | 0.703468 |
Target: 5'- gCGGCGCGCgGcgcgUUCAgcaugcuuuGUGcCGCGGCg -3' miRNA: 3'- aGCUGCGCGgCa---GAGU---------UACaGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 51669 | 0.71 | 0.632491 |
Target: 5'- aCGgguGCGUGUCGUCgcugacgCAGuUGUUGCGGCa -3' miRNA: 3'- aGC---UGCGCGGCAGa------GUU-ACAGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 123052 | 0.71 | 0.622285 |
Target: 5'- -gGGC-CGCCGg-UCGAgGUCGCGGCg -3' miRNA: 3'- agCUGcGCGGCagAGUUaCAGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 5248 | 0.73 | 0.531614 |
Target: 5'- --cACGCGCCG-CUCGAaacgCGCGGCg -3' miRNA: 3'- agcUGCGCGGCaGAGUUaca-GCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 69670 | 0.66 | 0.906136 |
Target: 5'- gCG-CGUGCCagaagagcuuGUCUCAAUua-GCGGCg -3' miRNA: 3'- aGCuGCGCGG----------CAGAGUUAcagCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 109397 | 0.66 | 0.893349 |
Target: 5'- uUCGACGCGCUGggugUUCAAagugacUGaCGCGuuGCa -3' miRNA: 3'- -AGCUGCGCGGCa---GAGUU------ACaGCGC--CG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 37709 | 0.66 | 0.886613 |
Target: 5'- cUUGACGUcacGCCGUUUCAagGUagcaaaaaccgGCGGCu -3' miRNA: 3'- -AGCUGCG---CGGCAGAGUuaCAg----------CGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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