Results 21 - 34 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22856 | 5' | -56 | NC_005137.2 | + | 111169 | 0.7 | 0.693437 |
Target: 5'- aCGG-GCGgCGgaggCUUuGUGUCGCGGCg -3' miRNA: 3'- aGCUgCGCgGCa---GAGuUACAGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 113693 | 0.7 | 0.693437 |
Target: 5'- gCGACGCGCaCGUCgcacagcgcgUCAAUGUCcUGcGCa -3' miRNA: 3'- aGCUGCGCG-GCAG----------AGUUACAGcGC-CG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 100531 | 0.69 | 0.739016 |
Target: 5'- gUCGACGCGCCGcCUCAAcucuauaaaacgaGcCGCGcaGCa -3' miRNA: 3'- -AGCUGCGCGGCaGAGUUa------------CaGCGC--CG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 63461 | 0.69 | 0.762074 |
Target: 5'- cUCGGCGCG-CGUUU--GUGccgccUCGCGGCg -3' miRNA: 3'- -AGCUGCGCgGCAGAguUAC-----AGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 73542 | 0.69 | 0.771494 |
Target: 5'- aCGGCGuCGUgGcCUCGuUG-CGCGGCg -3' miRNA: 3'- aGCUGC-GCGgCaGAGUuACaGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 71372 | 0.69 | 0.78079 |
Target: 5'- gCGGCGUGCCGUUgCAAaaa-GUGGCg -3' miRNA: 3'- aGCUGCGCGGCAGaGUUacagCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 26569 | 0.68 | 0.825109 |
Target: 5'- gCGGCGUGUCGaCUgccaaGAUGUacaCGCGGCu -3' miRNA: 3'- aGCUGCGCGGCaGAg----UUACA---GCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 118312 | 0.68 | 0.825109 |
Target: 5'- gUUGGCGCGCCGUUcCGAcG-CGCuGCu -3' miRNA: 3'- -AGCUGCGCGGCAGaGUUaCaGCGcCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 15728 | 0.67 | 0.841672 |
Target: 5'- cUCGuC-CGCUG-CUCGccaagGUCGCGGCg -3' miRNA: 3'- -AGCuGcGCGGCaGAGUua---CAGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 90496 | 0.67 | 0.849673 |
Target: 5'- cUCGAUuauaGCGCUgcuaGUUUCAAUGUCGCa-- -3' miRNA: 3'- -AGCUG----CGCGG----CAGAGUUACAGCGccg -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 25442 | 0.66 | 0.879653 |
Target: 5'- aCGAcaaCGCGuUUGUCcaCAAaGUCGCGGCa -3' miRNA: 3'- aGCU---GCGC-GGCAGa-GUUaCAGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 37709 | 0.66 | 0.886613 |
Target: 5'- cUUGACGUcacGCCGUUUCAagGUagcaaaaaccgGCGGCu -3' miRNA: 3'- -AGCUGCG---CGGCAGAGUuaCAg----------CGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 109397 | 0.66 | 0.893349 |
Target: 5'- uUCGACGCGCUGggugUUCAAagugacUGaCGCGuuGCa -3' miRNA: 3'- -AGCUGCGCGGCa---GAGUU------ACaGCGC--CG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 69670 | 0.66 | 0.906136 |
Target: 5'- gCG-CGUGCCagaagagcuuGUCUCAAUua-GCGGCg -3' miRNA: 3'- aGCuGCGCGG----------CAGAGUUAcagCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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