Results 21 - 34 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22856 | 5' | -56 | NC_005137.2 | + | 31464 | 0.69 | 0.752539 |
Target: 5'- aCaACGCGuuGUCUU-GUG-CGCGGCa -3' miRNA: 3'- aGcUGCGCggCAGAGuUACaGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 97431 | 0.69 | 0.742899 |
Target: 5'- -gGGCGCGgCGcaCUCAaaaacAUGUCGgGGCa -3' miRNA: 3'- agCUGCGCgGCa-GAGU-----UACAGCgCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 63461 | 0.69 | 0.762074 |
Target: 5'- cUCGGCGCG-CGUUU--GUGccgccUCGCGGCg -3' miRNA: 3'- -AGCUGCGCgGCAGAguUAC-----AGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 100531 | 0.69 | 0.739016 |
Target: 5'- gUCGACGCGCCGcCUCAAcucuauaaaacgaGcCGCGcaGCa -3' miRNA: 3'- -AGCUGCGCGGCaGAGUUa------------CaGCGC--CG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 113693 | 0.7 | 0.693437 |
Target: 5'- gCGACGCGCaCGUCgcacagcgcgUCAAUGUCcUGcGCa -3' miRNA: 3'- aGCUGCGCG-GCAG----------AGUUACAGcGC-CG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 111169 | 0.7 | 0.693437 |
Target: 5'- aCGG-GCGgCGgaggCUUuGUGUCGCGGCg -3' miRNA: 3'- aGCUgCGCgGCa---GAGuUACAGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 14114 | 0.7 | 0.693437 |
Target: 5'- gUGACG-GCCGUUgUCAA-GUCGCGGg -3' miRNA: 3'- aGCUGCgCGGCAG-AGUUaCAGCGCCg -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 100049 | 0.7 | 0.703468 |
Target: 5'- gCGGCGCGCgGcgcgUUCAgcaugcuuuGUGcCGCGGCg -3' miRNA: 3'- aGCUGCGCGgCa---GAGU---------UACaGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 51669 | 0.71 | 0.632491 |
Target: 5'- aCGgguGCGUGUCGUCgcugacgCAGuUGUUGCGGCa -3' miRNA: 3'- aGC---UGCGCGGCAGa------GUU-ACAGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 123052 | 0.71 | 0.622285 |
Target: 5'- -gGGC-CGCCGg-UCGAgGUCGCGGCg -3' miRNA: 3'- agCUGcGCGGCagAGUUaCAGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 73432 | 0.73 | 0.551453 |
Target: 5'- gCGGCGCGCUGggCUCGAcgcGUucuaaaCGCGGCg -3' miRNA: 3'- aGCUGCGCGGCa-GAGUUa--CA------GCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 85250 | 0.73 | 0.541503 |
Target: 5'- aUCGGCGuCGCUGUCgUCAGcggcguaacccuUGgCGCGGCu -3' miRNA: 3'- -AGCUGC-GCGGCAG-AGUU------------ACaGCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 5248 | 0.73 | 0.531614 |
Target: 5'- --cACGCGCCG-CUCGAaacgCGCGGCg -3' miRNA: 3'- agcUGCGCGGCaGAGUUaca-GCGCCG- -5' |
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22856 | 5' | -56 | NC_005137.2 | + | 125929 | 1.13 | 0.001601 |
Target: 5'- gUCGACGCGCCGUCUCAAUGUCGCGGCg -3' miRNA: 3'- -AGCUGCGCGGCAGAGUUACAGCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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