Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22857 | 3' | -51.8 | NC_005137.2 | + | 15751 | 0.68 | 0.9358 |
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Target: 5'- gCGGCGCCAGCGucgcCAUGugUucguCGACc- -3' miRNA: 3'- gGCCGUGGUCGUu---GUACugA----GUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 5958 | 0.68 | 0.940777 |
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Target: 5'- uCCGGCGCCGcGCcGCccGGCUCcgUUGc -3' miRNA: 3'- -GGCCGUGGU-CGuUGuaCUGAGuuGAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 32335 | 0.68 | 0.940777 |
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Target: 5'- gUGGC-CCAGCAGCAcGA-UCAGCUc -3' miRNA: 3'- gGCCGuGGUCGUUGUaCUgAGUUGAc -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 59315 | 0.67 | 0.958148 |
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Target: 5'- gCGGCACCAGCAccucGAUcCGGCUa -3' miRNA: 3'- gGCCGUGGUCGUuguaCUGaGUUGAc -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 69435 | 0.67 | 0.961875 |
|
Target: 5'- gCUGGCGgCGGCAauuauuaucauuACGUG-CUCAACg- -3' miRNA: 3'- -GGCCGUgGUCGU------------UGUACuGAGUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 49331 | 0.67 | 0.968624 |
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Target: 5'- cCCGaCACCGGCAGC--GAUUCGGCc- -3' miRNA: 3'- -GGCcGUGGUCGUUGuaCUGAGUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 68005 | 0.67 | 0.968624 |
|
Target: 5'- gCCGGCGCC-GCAcCAUGGgUCcGCc- -3' miRNA: 3'- -GGCCGUGGuCGUuGUACUgAGuUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 26561 | 0.66 | 0.977085 |
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Target: 5'- gUGGCGCU-GCGGCGUG--UCGACUGc -3' miRNA: 3'- gGCCGUGGuCGUUGUACugAGUUGAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 71319 | 0.66 | 0.983735 |
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Target: 5'- aCGGcCACgUAGCAACAUGAUaaGACa- -3' miRNA: 3'- gGCC-GUG-GUCGUUGUACUGagUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 123656 | 1.12 | 0.004051 |
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Target: 5'- gCCGGCACCAGCAACAUGACUCAACUGg -3' miRNA: 3'- -GGCCGUGGUCGUUGUACUGAGUUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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