Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22857 | 3' | -51.8 | NC_005137.2 | + | 31228 | 0.68 | 0.93931 |
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Target: 5'- gCGGCacACCAGCuugAACAUGgccgcgucgugcgcGCUCAACUc -3' miRNA: 3'- gGCCG--UGGUCG---UUGUAC--------------UGAGUUGAc -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 30804 | 0.66 | 0.982539 |
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Target: 5'- aCGcGCACCAGCAACAcguuggGAgUgugcgcguuuaauacCAACUGa -3' miRNA: 3'- gGC-CGUGGUCGUUGUa-----CUgA---------------GUUGAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 28897 | 0.7 | 0.864205 |
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Target: 5'- gUGGCGUCGGUGAUAUGGCUUAugUGc -3' miRNA: 3'- gGCCGUGGUCGUUGUACUGAGUugAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 26561 | 0.66 | 0.977085 |
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Target: 5'- gUGGCGCU-GCGGCGUG--UCGACUGc -3' miRNA: 3'- gGCCGUGGuCGUUGUACugAGUUGAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 25487 | 0.69 | 0.907037 |
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Target: 5'- aCGGUGCCAGCAGCcguGUGGCgCcGCUu -3' miRNA: 3'- gGCCGUGGUCGUUG---UACUGaGuUGAc -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 22394 | 0.75 | 0.652065 |
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Target: 5'- cCCuacGCACCGGCAACAUGGCgCGcCUGa -3' miRNA: 3'- -GGc--CGUGGUCGUUGUACUGaGUuGAC- -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 20575 | 0.68 | 0.949964 |
|
Target: 5'- aCCGaGgACCucGCGACAUGACcgUCGACg- -3' miRNA: 3'- -GGC-CgUGGu-CGUUGUACUG--AGUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 15751 | 0.68 | 0.9358 |
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Target: 5'- gCGGCGCCAGCGucgcCAUGugUucguCGACc- -3' miRNA: 3'- gGCCGUGGUCGUu---GUACugA----GUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 7364 | 0.69 | 0.925071 |
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Target: 5'- gCUGGUGCCGGCAaaauaGCGUGGgucUUCAGCg- -3' miRNA: 3'- -GGCCGUGGUCGU-----UGUACU---GAGUUGac -5' |
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| 22857 | 3' | -51.8 | NC_005137.2 | + | 5958 | 0.68 | 0.940777 |
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Target: 5'- uCCGGCGCCGcGCcGCccGGCUCcgUUGc -3' miRNA: 3'- -GGCCGUGGU-CGuUGuaCUGAGuuGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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