Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22868 | 5' | -56.1 | NC_005137.2 | + | 19607 | 0.66 | 0.894527 |
Target: 5'- aACACa--GGgGCGGCCGCuGCGGCCu -3' miRNA: 3'- gUGUGcagCCaCGCUGGCG-UGUUGGu -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 12696 | 0.66 | 0.894527 |
Target: 5'- aGCGCGUCGGaaCGGCgCGC-CAACa- -3' miRNA: 3'- gUGUGCAGCCacGCUG-GCGuGUUGgu -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 98027 | 0.66 | 0.887778 |
Target: 5'- uGCACGUCGG-GCG-CUGCgaGCGgguCCAg -3' miRNA: 3'- gUGUGCAGCCaCGCuGGCG--UGUu--GGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 7245 | 0.66 | 0.883619 |
Target: 5'- aACGUGUUGGUggcaacguugaccucGCGACCuaACAACCGg -3' miRNA: 3'- gUGUGCAGCCA---------------CGCUGGcgUGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 31130 | 0.66 | 0.880801 |
Target: 5'- gAUGCGUCGGUGUacgaguuuGACgG-ACAGCCGg -3' miRNA: 3'- gUGUGCAGCCACG--------CUGgCgUGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 33728 | 0.66 | 0.880091 |
Target: 5'- aACACGUUGGcuacugcgGCGGCguuguaccgaaaaCGCAUGGCCAu -3' miRNA: 3'- gUGUGCAGCCa-------CGCUG-------------GCGUGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 112527 | 0.66 | 0.873601 |
Target: 5'- cUugACGUCGGcaCaGCCGCGCAcuauGCCAa -3' miRNA: 3'- -GugUGCAGCCacGcUGGCGUGU----UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 93641 | 0.66 | 0.873601 |
Target: 5'- aCGCAUGUUuGUGCGAcgacgaaaucuCCGC-CGGCCGc -3' miRNA: 3'- -GUGUGCAGcCACGCU-----------GGCGuGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 68755 | 0.66 | 0.866182 |
Target: 5'- aACGCGUUGGUGCcGCUugugugGCGCGaaaACCGu -3' miRNA: 3'- gUGUGCAGCCACGcUGG------CGUGU---UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 93460 | 0.67 | 0.850713 |
Target: 5'- gCAUACGggcaCGGUGCGcGCCaGCGCcguuuGCCGa -3' miRNA: 3'- -GUGUGCa---GCCACGC-UGG-CGUGu----UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 95899 | 0.67 | 0.850713 |
Target: 5'- --uGCGUCGcG-GUGACCGUACcguuGCCAa -3' miRNA: 3'- gugUGCAGC-CaCGCUGGCGUGu---UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 119839 | 0.67 | 0.850713 |
Target: 5'- gACuuCGUUGGgaucgggcgugGUGGCaCGCGCAGCCAa -3' miRNA: 3'- gUGu-GCAGCCa----------CGCUG-GCGUGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 129331 | 0.67 | 0.850713 |
Target: 5'- uGCGCG-CGuUGCuGACCGCGCcgaAACCGa -3' miRNA: 3'- gUGUGCaGCcACG-CUGGCGUG---UUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 107971 | 0.67 | 0.842675 |
Target: 5'- uGCGCGaUCGGUcGCGuugacGCgCGCGCAGCUu -3' miRNA: 3'- gUGUGC-AGCCA-CGC-----UG-GCGUGUUGGu -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 109406 | 0.67 | 0.831098 |
Target: 5'- aCAC-CGUCGGgcucgucaaaaccGCGGCCuauGCGCGGCCc -3' miRNA: 3'- -GUGuGCAGCCa------------CGCUGG---CGUGUUGGu -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 22460 | 0.68 | 0.808665 |
Target: 5'- gCGCucCGUCGGcUGC-ACCGCGCucacGCCGg -3' miRNA: 3'- -GUGu-GCAGCC-ACGcUGGCGUGu---UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 31725 | 0.68 | 0.808665 |
Target: 5'- -uCGCGUCGuuucGCGugCGUAUAGCCGc -3' miRNA: 3'- guGUGCAGCca--CGCugGCGUGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 20375 | 0.68 | 0.808665 |
Target: 5'- aCGC-CGUCGGcGCGuuugUGCACGGCCGu -3' miRNA: 3'- -GUGuGCAGCCaCGCug--GCGUGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 102272 | 0.68 | 0.799738 |
Target: 5'- gACGCGUac--GCGACCGCAauaguCAACCAc -3' miRNA: 3'- gUGUGCAgccaCGCUGGCGU-----GUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 129504 | 0.68 | 0.799738 |
Target: 5'- aACGCGUCG--GCG-CCGCGCGGCgGa -3' miRNA: 3'- gUGUGCAGCcaCGCuGGCGUGUUGgU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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