Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22868 | 5' | -56.1 | NC_005137.2 | + | 99311 | 1.08 | 0.003058 |
Target: 5'- gCACACGUCGGUGCGACCGCACAACCAc -3' miRNA: 3'- -GUGUGCAGCCACGCUGGCGUGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 75976 | 0.77 | 0.335402 |
Target: 5'- gCGCGCGUUGGUGC-ACgGCGCAcggGCCAu -3' miRNA: 3'- -GUGUGCAGCCACGcUGgCGUGU---UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 67338 | 0.74 | 0.444385 |
Target: 5'- aACGCGUCGGguucgGUGACCGCgggcGCGACgCGa -3' miRNA: 3'- gUGUGCAGCCa----CGCUGGCG----UGUUG-GU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 18610 | 0.73 | 0.491283 |
Target: 5'- gGCGCGcCGuGcGCGAUCGCGCAAUCAa -3' miRNA: 3'- gUGUGCaGC-CaCGCUGGCGUGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 99947 | 0.72 | 0.580743 |
Target: 5'- aCGCAUGUUGGacaacggccGCGGCCGCGCGccucuucgugcGCCAg -3' miRNA: 3'- -GUGUGCAGCCa--------CGCUGGCGUGU-----------UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 118103 | 0.7 | 0.662838 |
Target: 5'- gACGCGUgCGGUGCaacaagGugCGCACcguGACCGa -3' miRNA: 3'- gUGUGCA-GCCACG------CugGCGUG---UUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 18310 | 0.69 | 0.743274 |
Target: 5'- -cCGCG-CGGgcaucGCGACUGCACGgacGCCAa -3' miRNA: 3'- guGUGCaGCCa----CGCUGGCGUGU---UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 97993 | 0.69 | 0.733465 |
Target: 5'- gCGCgGCGUgGGUGCGcCCGC-CAagugGCCAg -3' miRNA: 3'- -GUG-UGCAgCCACGCuGGCGuGU----UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 95881 | 0.69 | 0.752984 |
Target: 5'- gGCGCGugucaaaccuUCGGUGuUGACCGCguuugcgccaaACAGCCGc -3' miRNA: 3'- gUGUGC----------AGCCAC-GCUGGCG-----------UGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 106695 | 0.69 | 0.713591 |
Target: 5'- uGCGCGgacgCGGUG-GACUGCAacaAGCCGu -3' miRNA: 3'- gUGUGCa---GCCACgCUGGCGUg--UUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 72795 | 0.68 | 0.762587 |
Target: 5'- cCACGCGUUG--GCGGCgCGCGCuGCCGu -3' miRNA: 3'- -GUGUGCAGCcaCGCUG-GCGUGuUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 75129 | 0.68 | 0.790658 |
Target: 5'- gGCGuCGUCGGcGCGAUUGUugGacGCCAa -3' miRNA: 3'- gUGU-GCAGCCaCGCUGGCGugU--UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 102272 | 0.68 | 0.799738 |
Target: 5'- gACGCGUac--GCGACCGCAauaguCAACCAc -3' miRNA: 3'- gUGUGCAgccaCGCUGGCGU-----GUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 22460 | 0.68 | 0.808665 |
Target: 5'- gCGCucCGUCGGcUGC-ACCGCGCucacGCCGg -3' miRNA: 3'- -GUGu-GCAGCC-ACGcUGGCGUGu---UGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 31725 | 0.68 | 0.808665 |
Target: 5'- -uCGCGUCGuuucGCGugCGUAUAGCCGc -3' miRNA: 3'- guGUGCAGCca--CGCugGCGUGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 62269 | 0.68 | 0.799738 |
Target: 5'- aACGCGUUGGU-CGACgGCA--ACCAa -3' miRNA: 3'- gUGUGCAGCCAcGCUGgCGUguUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 129504 | 0.68 | 0.799738 |
Target: 5'- aACGCGUCG--GCG-CCGCGCGGCgGa -3' miRNA: 3'- gUGUGCAGCcaCGCuGGCGUGUUGgU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 20375 | 0.68 | 0.808665 |
Target: 5'- aCGC-CGUCGGcGCGuuugUGCACGGCCGu -3' miRNA: 3'- -GUGuGCAGCCaCGCug--GCGUGUUGGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 100614 | 0.68 | 0.772073 |
Target: 5'- aCAUACGU-GGUaaacGCGugCGCACAGgCAc -3' miRNA: 3'- -GUGUGCAgCCA----CGCugGCGUGUUgGU- -5' |
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22868 | 5' | -56.1 | NC_005137.2 | + | 66471 | 0.68 | 0.789742 |
Target: 5'- gCGCACGUUGGUcaacguuGCcgGugCGCACAcGCCGu -3' miRNA: 3'- -GUGUGCAGCCA-------CG--CugGCGUGU-UGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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