Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22871 | 5' | -54.1 | NC_005137.2 | + | 119567 | 0.66 | 0.93624 |
Target: 5'- -cUCGGUAgAGCGaCGCaCGCug-AGCa -3' miRNA: 3'- aaAGCCAUgUUGCgGCG-GCGucaUCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 12067 | 0.66 | 0.93624 |
Target: 5'- -gUCGGUcgGCAAucacguCGCCGaCCGCAaGU-GCg -3' miRNA: 3'- aaAGCCA--UGUU------GCGGC-GGCGU-CAuCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 24300 | 0.66 | 0.931078 |
Target: 5'- --aCGGcgGCGuuUGCgGCUGCGGUGGUg -3' miRNA: 3'- aaaGCCa-UGUu-GCGgCGGCGUCAUCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 677 | 0.66 | 0.931078 |
Target: 5'- --cUGGUcaaACAACG-CGCUGCgaucGGUAGCg -3' miRNA: 3'- aaaGCCA---UGUUGCgGCGGCG----UCAUCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 66477 | 0.66 | 0.925662 |
Target: 5'- -gUUGGU-CAACGuuGCCGguGc-GCa -3' miRNA: 3'- aaAGCCAuGUUGCggCGGCguCauCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 73118 | 0.66 | 0.919991 |
Target: 5'- -gUCcuGUACGGCGUCGUCGUAGUcGUc -3' miRNA: 3'- aaAGc-CAUGUUGCGGCGGCGUCAuCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 74331 | 0.66 | 0.91941 |
Target: 5'- --cCGGUcacguccACAGCGCCGUCgGCA--AGCa -3' miRNA: 3'- aaaGCCA-------UGUUGCGGCGG-CGUcaUCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 97773 | 0.67 | 0.90789 |
Target: 5'- --aCGGUgagucgGCGGCGUuaaacugguucuCGCCGCGGUGcGCu -3' miRNA: 3'- aaaGCCA------UGUUGCG------------GCGGCGUCAU-CG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 76200 | 0.67 | 0.90789 |
Target: 5'- uUUUUGGUG--GCGCuuuuuauguaCGuuGCGGUAGCg -3' miRNA: 3'- -AAAGCCAUguUGCG----------GCggCGUCAUCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 1495 | 0.67 | 0.894788 |
Target: 5'- --cCGGUACAuu-CCGCCGCGcGgcGCc -3' miRNA: 3'- aaaGCCAUGUugcGGCGGCGU-CauCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 95482 | 0.67 | 0.894788 |
Target: 5'- --cCGGUcgGAUGCgCGCCGCAcgGGCg -3' miRNA: 3'- aaaGCCAugUUGCG-GCGGCGUcaUCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 61135 | 0.68 | 0.865693 |
Target: 5'- -gUUGGUGCaAACGUCGUCGCAaauuGCu -3' miRNA: 3'- aaAGCCAUG-UUGCGGCGGCGUcau-CG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 31676 | 0.68 | 0.857846 |
Target: 5'- --gCGG-ACGACaCCGCCGC-GUGGUu -3' miRNA: 3'- aaaGCCaUGUUGcGGCGGCGuCAUCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 76596 | 0.68 | 0.857846 |
Target: 5'- -cUCGuGUugGACGCCGCCGaaCAGa--- -3' miRNA: 3'- aaAGC-CAugUUGCGGCGGC--GUCaucg -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 89534 | 0.68 | 0.857846 |
Target: 5'- ---aGGU-CAACGuCCGCUGCGGacAGCg -3' miRNA: 3'- aaagCCAuGUUGC-GGCGGCGUCa-UCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 130188 | 0.69 | 0.824361 |
Target: 5'- --cUGGUGUAAacgUGCCGCCGCcaccuGUAGCg -3' miRNA: 3'- aaaGCCAUGUU---GCGGCGGCGu----CAUCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 27311 | 0.69 | 0.815505 |
Target: 5'- --aCGGUgGCGGCGgCGUUuuggGCAGUGGCg -3' miRNA: 3'- aaaGCCA-UGUUGCgGCGG----CGUCAUCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 63614 | 0.69 | 0.806471 |
Target: 5'- uUUUUGGaacaaaaauuCAACGCCGCCGCGGcgacGGUc -3' miRNA: 3'- -AAAGCCau--------GUUGCGGCGGCGUCa---UCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 2572 | 0.69 | 0.787909 |
Target: 5'- ---aGGUGCAGCGCauuaaCGCAgGUGGCg -3' miRNA: 3'- aaagCCAUGUUGCGgcg--GCGU-CAUCG- -5' |
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22871 | 5' | -54.1 | NC_005137.2 | + | 52226 | 0.7 | 0.7784 |
Target: 5'- --aCGGcACGuauacuCGUCGCCGCAGaAGCu -3' miRNA: 3'- aaaGCCaUGUu-----GCGGCGGCGUCaUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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