Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22873 | 3' | -49.1 | NC_005137.2 | + | 101868 | 0.71 | 0.949222 |
|
Target: 5'- aCGcAGCaauuguUUUGUCu---UGGCGCCGGCc -3' miRNA: 3'- -GC-UCGc-----AAAUAGuuuuACCGCGGCCG- -5' |
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| 22873 | 3' | -49.1 | NC_005137.2 | + | 104764 | 0.67 | 0.99561 |
|
Target: 5'- gGGGCG-UUGUUAAcguuccacgagguucGGUGGCccaGUCGGCg -3' miRNA: 3'- gCUCGCaAAUAGUU---------------UUACCG---CGGCCG- -5' |
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| 22873 | 3' | -49.1 | NC_005137.2 | + | 113667 | 0.66 | 0.997605 |
|
Target: 5'- -uAGUGUacuauuuaUAUCAGAGUGGUGCgCGuGCg -3' miRNA: 3'- gcUCGCAa-------AUAGUUUUACCGCG-GC-CG- -5' |
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| 22873 | 3' | -49.1 | NC_005137.2 | + | 116340 | 0.67 | 0.996014 |
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Target: 5'- aCGGGCGUUUacGUCAAGcuuaaacgauuuGUGcGCGCUGa- -3' miRNA: 3'- -GCUCGCAAA--UAGUUU------------UAC-CGCGGCcg -5' |
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| 22873 | 3' | -49.1 | NC_005137.2 | + | 128560 | 0.68 | 0.990262 |
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Target: 5'- aGAGUG---GUCGucg-GGCGCgGGCa -3' miRNA: 3'- gCUCGCaaaUAGUuuuaCCGCGgCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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