Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22886 | 3' | -53.6 | NC_005137.2 | + | 112457 | 0.67 | 0.876897 |
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Target: 5'- cGCGgcgGCAGGAUggGCGaCGGCGCc- -3' miRNA: 3'- aCGCaagCGUUCUAa-CGC-GUCGCGuu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 78153 | 0.67 | 0.884321 |
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Target: 5'- -aCGUUUGCAaauAGAUUGCGuCAcGCGCu- -3' miRNA: 3'- acGCAAGCGU---UCUAACGC-GU-CGCGuu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 110712 | 0.67 | 0.884321 |
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Target: 5'- cGCGUgCGCAAcGGUgUGCGCgcagAGCGCu- -3' miRNA: 3'- aCGCAaGCGUU-CUA-ACGCG----UCGCGuu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 58656 | 0.66 | 0.911503 |
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Target: 5'- aGCGUgcaCGC-GGAgcgGCGCAgcucGCGCAAu -3' miRNA: 3'- aCGCAa--GCGuUCUaa-CGCGU----CGCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 124928 | 0.66 | 0.917048 |
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Target: 5'- cGCGUugaugugcuugaUCGCcGGGUUGCgaugaacGCGGCGCc- -3' miRNA: 3'- aCGCA------------AGCGuUCUAACG-------CGUCGCGuu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 74753 | 0.66 | 0.917651 |
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Target: 5'- -cCGUU-GCAAcAUUGCGCGGUGCGg -3' miRNA: 3'- acGCAAgCGUUcUAACGCGUCGCGUu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 75584 | 0.66 | 0.923536 |
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Target: 5'- gGCGgaCGCGGGcg-GCGCcguGCGCGu -3' miRNA: 3'- aCGCaaGCGUUCuaaCGCGu--CGCGUu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 25684 | 0.66 | 0.929156 |
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Target: 5'- gGCGUUUGCAgacaagguGGAUUGggaCGCGGUGUc- -3' miRNA: 3'- aCGCAAGCGU--------UCUAAC---GCGUCGCGuu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 113177 | 0.66 | 0.934512 |
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Target: 5'- uUGCGUUCGUAAGGU--CGaCGGCGg-- -3' miRNA: 3'- -ACGCAAGCGUUCUAacGC-GUCGCguu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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